Re: GFAP and NeuN on mouse tissue?

From:Matt McElwee

Hi Melissa.  I've started using Chemicon's mouse anti-NeuN antibody and
gotten good results with perfusion fixed vibratome sections.  I wouldn't
cut any thicker than 40 microns.  We use a 1:1000 antibody dilution
incubated for 48 hours.  Let me know if you have any more questions.

Matt McElwee
Research Specialist
Department of Surgery
K4/617 Clinical Science Center
600 Highland Avenue
Madison, WI 53792
(608) 263-7648

>>> Melissa Gonzalez  02/28/03
06:03PM >>>
Does anyone have any experience using Chemicon's mouse anti-NeuN and/or
DAKO's rabbit anti cow GFAP?
I have seen a few discussions relating to FFPE human tissue, but we are
working on mouse frozens for fluorescence microscopy..which leads into
my next question, How thick can I keep (or how thin should I cut) the
tissue (brain) for optimal visualization?

Thanks in advance for your help!

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