Re: FWD'ing a post for Ronnie Houston RE c-kit

From:Patti Loykasek

We have been routinely using the ckit from Dako, and have not seen this type
of staining. We have compared this antibody to a couple of other clones, and
it has continuously come out on top. I am wondering if perhaps either
tissues fixation/processing is affecting the staining. Or perhaps it is a
heat pretreatment or titer issue. Just my thoughts.

Patti Loykasek
Phenopath Laboratories
Seattle, WA>

 One of our pathologists has shared some information he was given by a
> at AFIP. I have never seen this before, but was wondering if anyone else
> has.
> They claim that the c-Kit antibody from Dako(A4502)gives "widespread
> positivty of fibroblasts and myofibroblasts, suggesting non-specific cross
> reactivity with this particular antibody". However, they did not experience
> this phenomenon with the c-kit from Santa Cruz Biotechnology (sc-168). Dako
> are not aware of any issues with their antibody.
> Both of these are polyclonals.
> Appreciate your input
> Thanks
> Ronnie 
> Ronnie Houston
> Regional Histology Operations Manager
> Bon Secours HealthPartners Laboratories
> 5801 Bremo Road
> Richmond, VA 23226
> (804) 287 7972

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