Re: FWD'ing a post for Ronnie Houston RE c-kit

From:Lisa Black

I purchased the c-Kit from Santa Cruz and could never get good results. 
What are you using for control and what is your protocol?
Thanks
Lisa Black
Carilion Consolidated Laboratory
Histology Supervisor

>>> Carrie Kyle-Byrne  3/13/03 4:18:23 PM >>>
One of our pathologists has shared some information he was given by a
doctor
at AFIP. I have never seen this before, but was wondering if anyone
else
has.

They claim that the c-Kit antibody from Dako(A4502)gives "widespread
positivty of fibroblasts and myofibroblasts, suggesting non-specific
cross
reactivity with this particular antibody". However, they did not
experience
this phenomenon with the c-kit from Santa Cruz Biotechnology (sc-168).
Dako
are not aware of any issues with their antibody.

Both of these are polyclonals.

Appreciate your input
Thanks
Ronnie 

Ronnie Houston
Regional Histology Operations Manager
Bon Secours HealthPartners Laboratories
5801 Bremo Road
Richmond, VA 23226
(804) 287 7972
ronnie_houston@bshsi.com 






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