Actually it is in JOH Sept. 1998

Gayle Callis wrote:

>Tungsten Carbide knife - embedded in PMMA for microtomed sections, but
>teeth must be fixed, PMMA can be removed BEFORE staining - IHC would
>require stringent antigen retrieval - Neil Hand has excellent retrieval
>method published in J Histotechnology in 1999 or so. 
>
>For frozen sections, more difficult to get a good section without
>Instrumedics Cryojane tape transfer plus you need to use a tungsten carbide
>blade, d profile
>
>You may have results using EDTA decalcification and cut in cryostat.  This
>can even be done on fresh unfixed or unfixed bone/teeth, fix frozen
>sections before staining. 
>
>Check out J of Histochemistry Cytochemistry, a recent publication did a
>similar method using PLP fixation, etc. 
>
>Also Mori S et al J Histochem Cytochem, 36:111-114, 1988 did EDTA on
>unfixed fresh bone for cell surface markers. 
>
>Not an easy project!
> 
>
>
>
>Gayle Callis
>MT,HT,HTL(ASCP)
>Research Histopathology Supervisor
>Veterinary Molecular Biology - Marsh Lab
>Montana State University - Bozeman
>S. 19th and Lincoln St
>Bozeman MT 59717-3610
>
>406 994-6367 (lab with voice mail)
>406 994-4303 (FAX)
>
>email: gcallis@montana.edu
>
>
>  
>