Re: 40 um paraffin sections coming loose from the slides

From:Paula Fisher

Try Menzel's Superfrost Plus slides that are positively charged.  They are
fairly expensive, but they are very high quality and we haven't seen the
problem of sections floating off since we used them.  However, I have never
cut sections this thick.  It is worth a try.
Best regards,

----- Original Message -----
From: "Susanne S#248#rensen" 
To: "histonet" 
Sent: Thursday, March 06, 2003 8:53 AM
Subject: 40 um paraffin sections coming loose from the slides

Hi Everyone

 I'm cutting 40 um paraffin sections, human brain, so the sections is very
big. The problem is, that some sections (to much) is coming loose from the
slides when I rehydrate. I coated the slides with 4,5% gelatine and 4,0%
potassium chromic sulphate.  I have tried silane slides but the
gelatine/ChromeAlun seems to be the best for now.

If anyone has a suggestion to overcome the problem with loose section, I
would like to here from you.


Sus S#248#rensen
Research Lab Stereology & Neuroscience

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