RE: storage frozen tissue -80C

From:Pamela Marcum

Do you really think he should stain NOW?  Actually I agree I have had
several of the possible accidents you mention happen to material I was
attempting to save over the years.  The freezer going out on a weekend when
the security guards forgo to patrol the far end of the hall and see what the
alarm going off meant was my personal favorite.  The emergency generator did
not kick in and five labs lost tissue samples collected over years.  None of
it was recoverable  and no said anything for days that could be repeated in
civilized culture.  I have had material thrown out by well meaning blank
comment and used without asking.  Anything I could finish and photograph I
did. In point of fact still do.  Paranoia is a wonderful thing.    Pam

> -----Original Message-----
> From: Geoff McAuliffe [mailto:mcauliff@UMDNJ.EDU]
> Sent: Wednesday, March 05, 2003 11:57 AM
> To: Frouwke Kuijpers
> Cc: histonet
> Subject: Re: storage frozen tissue -80C
>     If you can stain the slides now, do it. Why wait? Avoid all
> of the storage
> problems. Even after "perfect" storage (whatever that is) what if
> the freezer
> breaks down over the weekend? What if someone throws your material out by
> mistake? Or spills something on it? Why take chances with
> vaulable specimens.
>     Whether to store "wet" or "dry" probably depends on what you
> are looking
> for. You could store tissue both ways but what if neither way is
> good for what
> you are looking for?? Did I mention staining the material now?
> Geoff
> Frouwke Kuijpers wrote:
> > Hallo everybody
> >
> > I have a question about storage of frozen tissue.
> > We have no experience with that and we are forced to it now
> because the mice
> > which we use for our experiments are sick and it takes at least a year
> > before we can get new ones. So we have to be very economical
> with the tissue
> > we have left. We have the fixed sections now in autoclaved PBS
> and So-azide
> > at 4 C.
> >
> > People suggested we can store the left sections at - 80 C after mounting
> > them on slides. I have read about it in the archives of the Histonet and
> > most people advice to put silicagel in the slide boxes to prevent the
> > sections become too humid. Is that for the ice crystals?
> > But now my problem: other people of our University told me to
> put wet tissue
> > in the slideboxes, to prevent the sections are drying out? (Those white
> > spots you see when you left your frozen meat too long in the fridge).
> >
> > So, I am in a dilemma now, I have two complete opposite
> advices, what should
> > I do??
> >
> > Frouwke Kuijpers
> >
> > F.J.Kuijpers-Kwant
> > Dept. Cellular Animal Physiology
> > University of Nijmegen
> > Toernooiveld 1
> > 6525 ED Nijmegen
> >
> --
> **********************************************
> Geoff McAuliffe, Ph.D.
> Neuroscience and Cell Biology
> Robert Wood Johnson Medical School
> 675 Hoes Lane, Piscataway, NJ 08854
> voice: (732)-235-4583; fax: -4029
> **********************************************

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