RE: mouse lung CD3 immunostain

From:Jeff Gordon

Nick, just to let you know, Cell Marque's Ultramarque CD3 works on mouse tissue.  We discovered this from feedback that we got from NIH, which is using our CD3 both in the clinical hematology lab AND in the research lab by Dr. Matthew Starost, who is also testing it on other species as well.  This opens new possiblities for us to be able to market it to research labs as well as clinical labs.  We have been getting outstanding feedback from clients using our CD3 both for the quality of the stain and for the cost efficiency of the antibody with its super high titer (some labs are running it at 1:1000 with standard pretreatments).  If you would like to try it out, please let me know and I will get you a sample to evaluate.
Jeff Gordon
Cell Marque Corp.
-----Original Message-----
From: []
Sent: Tuesday, March 04, 2003 4:13 PM
To: Russ Ellis
Subject: Re: mouse lung CD3 immunostain

We use the Serotec Rat anti Human CD-3, and have used DAKO Retrieval Solution as well as Cell Marque Declere and Trilogy all with good results using the microwave or pressure cooker.  The pressure cooker was better though.  We used the Vector DAB kit and found that if we used the Nickel it was difficult to see the T Cells, but fairly easy using standard DAB, no frills(using THIS antibody).

Russ Ellis <>

03/04/03 01:53 PM

        Subject:        mouse lung CD3 immunostain

Hello everyone

I am developing a method for staining formalin-fixed,
paraffin-embedded mouse lungs with an antibody to CD3.
After an initial review of the archives I was hoping
for some feedback with the following:

1. ANTIBODY - Dako, Serotec, Novocastra, other(?).
I'm sure they all work to some degree, but what are
some of the negative aspects?

2. CITRATE BUFFER - should I be concerned about the
length of microwaving time given that lungs are more
fragile than most other tissues?

3.DAKO ARK - does anyone have any experience with this
"time saving" kit that they could provide some pros
and cons?  For me, time isn't a factor if the results
are poor.

I look forward to your useful insights.  If anyone
knows of a method that they can point me to as a place
to start, I would be very grateful.

Russ Ellis

Russ Ellis, B.Sc., M.L.T.
Research Lab Coordinator
St. Joseph's Hospital
Hamilton, Canada

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