RE: mouse lung CD3 immunostain
Nick,
just to let you know, Cell Marque's Ultramarque CD3 works on mouse tissue. We
discovered this from feedback that we got from NIH, which is using our CD3 both
in the clinical hematology lab AND in the research lab by Dr. Matthew
Starost, who is also testing it on other species
as well. This opens new possiblities for us to be able to market it
to research labs as well as clinical labs. We have been getting
outstanding feedback from clients
using our CD3 both for the quality of the stain and for the cost
efficiency of the antibody with its super high titer (some labs are running it
at 1:1000 with standard pretreatments). If you would like to try it out,
please let me know and I will get you a sample to
evaluate.
Jeff Gordon
Cell Marque Corp.
1-800-665-7284
We use the
Serotec Rat anti Human CD-3, and have used DAKO Retrieval Solution as well as
Cell Marque Declere and Trilogy all with good results using the microwave or
pressure cooker. The pressure cooker was better though. We used
the Vector DAB kit and found that if we used the Nickel it was difficult to
see the T Cells, but fairly easy using standard DAB, no frills(using THIS
antibody).
| Russ Ellis
<techmanruss@yahoo.ca>
03/04/03 01:53 PM
| To:
histonet@pathology.swmed.edu cc:
Subject: mouse lung CD3
immunostain |
Hello
everyone
I am developing a method for staining
formalin-fixed,
paraffin-embedded mouse lungs with an antibody to
CD3.
After an initial review of the archives I was hoping
for some
feedback with the following:
1. ANTIBODY - Dako, Serotec, Novocastra,
other(?).
I'm sure they all work to some degree, but what are
some of
the negative aspects?
2. CITRATE BUFFER - should I be concerned about
the
length of microwaving time given that lungs are more
fragile than
most other tissues?
3.DAKO ARK - does anyone have any experience with
this
"time saving" kit that they could provide some pros
and cons?
For me, time isn't a factor if the results
are poor.
I look
forward to your useful insights. If anyone
knows of a method that
they can point me to as a place
to start, I would be very
grateful.
Regards,
Russ Ellis
=====
Russ Ellis, B.Sc.,
M.L.T.
Research Lab Coordinator
St. Joseph's Hospital
Hamilton,
Canada
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