RE: microwaving slides

From:"Morken, Tim"

Microwaving slides was standard procedure in a lab I worked in previously. We would typically put several (up to 10) racks of slides in at once. The only problem I noticed was uneven melting amoung all the racks, but it seemed to work out fine for H&E's, however I would use a standard oven for my immuno and ISH slides simply to ensure even drying and melting.
Tim Morken
-----Original Message-----
From: []
Sent: Tuesday, March 04, 2003 7:08 PM
Subject: microwaving slides

Greetings to all,
I recently experimented microwaving slides prior to deparaffinization and staining.  I have searched the histonet archives to get a feel for what others think of doing this and if any problems were encountered during the process.  It seemed only one person was strongly against microwaving slides.

I want to cover my bases, so to speak, because I can see how this thing is taking off in our lab.  A few trial runs and people have quickly caught on to this as an option to expedite morning stains and trailing slides needing to make a hospital pickup.  I currently microwave anywhere between 1 to 10 slides at one time for 2 minutes at high power.  Has anyone noticed any difference between slides dried in this manner and slides dried by conventional oven?  It also my understanding that the paraffin doesn't melt but the heated water on the slide that melts that wax.  As long as the tissue stays on the slide and is deparaffinized completely through xylene, alcohols and this okay and accurate thinking?  One last nagging question:  Did those of you who started your own process of microwaving slides show the slides to doctors to ensure staining is still good even after microwaving?  

Deb King, HT
Sacramento, CA

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