RE: haemoglobin staining
From: | "Monson, Frederick C." |
Morning Louise,
Lillie (1965), p. 389, recommends a "Dunn-Thompson Hemoglobin Stain"
(Arch Path, 39:49, 1945) that goes as follows:
1. Stain 15 min in Mallory's alum hematoxylin (or likely any
non-acid hematoxylin prep)
2. Wash tap HOH
3. Mordant 1 min in 4% iron alum [lavender crystals only, please!]
4. Rinse in tap water [to remove excess]
5. Stain 15 min in picro-fuchsin solution;
a. 13 ml 1% acid fuchsin
b. 87 ml sat'd aqueous picric acid [bummer!]
6. dehydrate and differentiate 3 min in 1 change or more of 95%
EtOH. dehydrate with 100 EtOH (2 changes)
7. clear in xylene
8. mount in synthetic resin
hemoglobin everywhere-emerald green [perfect color for the moment]
I used this years ago, I believe with Delafield's, and it worked nicely.
Cheers,
Fred Monson
Frederick C. Monson, PhD
Center for Advanced Scientific Imaging
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-----Original Message-----
From: louise renton [mailto:louise_renton@hotmail.com]
Sent: Thursday, March 13, 2003 7:58 AM
To: histonet@pathology.swmed.edu
Subject: haemoglobin staining
Hello histofolks,
I am looking for a protocol for staining haemoglobin in FFPE* tissue. I
recall that there is a technique that uses benzidine, but given the toxicity
of this substance, i would prefer an alternative. Thank you in advance.
bets regards
* formalin fixed paraffin embedded
Louise Renton
Bone Research Unit
MRC
Johannesburg
South Africa
Tel & fax +27 11 717 2298
"Time flies like an arrow, fruit flies like a banana"
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