RE: beta galactosidase immunos

From:"Johnson, Teri"

Would this blocker also be effective in blocking non-specific staining in sebaceous glands in skin?

Teri Johnson
Managing Director Histology Facility
Stowers Institute for Medical Research
1000 E. 50th St.
Kansas City, Missouri  64110

-----Original Message-----
From: Demirs, John T. [mailto:DEMIRS@HELIX.MGH.HARVARD.EDU]
Sent: Monday, March 10, 2003 3:24 PM
To: Histo Net (E-mail)
Subject: RE: beta galactosidase immunos

Is this a monoclonal antibody, if so you need a mouse on mouse kit. Dako ARK
or Immungen work well. If it is a ployclonal then were is the background?
i.e. Glands, epidermis, dermis, etc. You could try Avidin/Biotin blockers,
or a universal blocker like Biogenex Power block, this is an enzyme blocker
and is very strong. 

John T. Demirs B. A. HTL (ASCP)
Wellman Laboratories of Photomedicine
Massachusetts General Hospital
Photopathology Dept. Edwards 214
15 Fruit St.
Boston Ma 02114
617-726-6984 phone
617-726-1206 fax

> -----Original Message-----
> From:	Martin, Ronald []
> Sent:	Monday, March 10, 2003 3:58 PM
> To:
> Subject:	beta galactosidase immunos
> Fellow netters,
> I am attempting beta gal immunos on mouse tissue and getting a lot of
> background stain. I have tried 3% H202 as is and made up from 30% H2O2 in
> methanol. I have also tried different antibody titration's from 1:200 up
> to 1:4000. I am redoing the 1:2000 and 1:3000 tomorrow but need some
> suggestions on how to reduce the background staining. I also use the
> recommended 3% goat serum.
> Thanks,
> Ron Martin

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