RE: Romulin AEC

From:Chris van der Loos

Dear Marilyn,
We have some brief experience with Romulin AEC. Indeed this type of AEC can 
be mounted up successfully with organic mounted media such as DEPEX, etc. 
However, it disappears after a couple of weeks when mounting with 
VectaMount, an organic but non-xylene or alcohol containing mounting medium.
The efficiency/sensitivity of the chromogen is nearly equal to our protocol 
for "regular" AEC (this protocol usually yields more reaction product than 
most commercially available AEC kit systems)
There is also a possibility to boost the efficiency/sensitivity by adding 
twice the amount of Romulin AEC chromogen.
A disadvantage of Romulin AEC is the color of the reaction product. In 
comparison with nice brick-red of "regular"AEC, Romulin AEC yields a 
brown-reddish color that is not so suitable for double staining with a blue 
color for example.

Chris van der Loos
Dept. of Cardiovascular Pathology
Academic Medical Center
Amsterdam, The Netherlands

Marilyn Johnson wrote:
 >Date: 10 Mar 2003 17:01:35 -0600
 >Subject: Romulin AEC
 >Hi Histonetters,
 >Are any Immunohistochemistry Labs. using the new Romulin AEC as the
 >chromogen? Advantages/disadvantages would be beneficial. We are looking
 >for alternate permanent chromogens other than DAB.
 >Thanks in advance for all replies.
 >Marilyn Johnson
 >Alberta Agriculture

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