Dako is your best bet. If they don't have what you want then I go to secondary
sources,no order of preference here, B D Pharmingen, Biogenex, BD biosciences,
R&D sciences, Novo castra. 
Try this web site, click on "Antibody suppliers" , you'll have over 200 to
choose from, http://www.antibodyresource.com/

John T. Demirs B. A. HTL (ASCP)
Wellman Laboratories of Photomedicine
Massachusetts General Hospital
Photopathology Dept. Edward 214
15 Fruit St.
Boston Ma 02114
617-726-6984 phone
617-726-1206 fax

> -----Original Message-----
> From:	NIDAL E MUVARAK [SMTP:nmuvarak@facstaff.wisc.edu]
> Sent:	Sunday, March 02, 2003 8:04 PM
> To:	Demirs, John T.
> Subject:	Re: RE: Immunostain for PCNA
> 
> Thanks for confiming my thoughts. I have repeatedly tried to tell my PI that
> Santa Cruz primaries are not the best, but she doesn't want to switch to
> anything else since her old tech spent a month trying to figure out the right
> dilution ( I looked at some of his slides and none of them impressed me). Just
> out of curiousity, where do you get your primary for PCNA from? Thanks.
> 
> Nidal E Muvarak
> Associate Research Specialist
> Department of Biomedical Engineering
> University of Wisconsin-Madison
> 1550 Engineering Dr.; Rm. 2158
> Madison, WI 53706-1609
> Lab: (608) 265-8921; Office: (608) 265-4205; 
> Home: (608) 256-7934; Cell: (608) 332-6068
> 
> ----- Original Message -----
> From: "Demirs, John T." 
> Date: Friday, February 28, 2003 1:28 pm
> Subject: RE: Immunostain for PCNA
> 
> > My first reactions is not to use Ab from Santa Cruze. I have never 
> > found any of
> > their primaries to be any good. 
> > Having said that, We use overnight incubations at 4C, tittered out to
> > 1:200-1:500. You should be doing Antigen Retrieval for nuclear 
> > staining and for
> > controls any tissue should have some level of cell proliferation 
> > some are better
> > then others i.e. liver, spleen, skin. 
> > John T. Demirs B. A. HTL (ASCP)
> > Wellman Laboratories of Photomedicine
> > Massachusetts General Hospital
> > Photopathology Dept. Edward 214
> > 15 Fruit St.
> > Boston Ma 02114
> > 617-726-6984 phone
> > 617-726-1206 fax
> > 
> > > -----Original Message-----
> > > From:	NIDAL E MUVARAK [SMTP:nmuvarak@facstaff.wisc.edu]
> > > Sent:	Thursday, February 27, 2003 3:14 PM
> > > To:	HistoNet Server
> > > Subject:	Immunostain for PCNA
> > > 
> > > Hi. I was wondering if anyone has any suggestions on a protocol 
> > for PCNA
> > > stain. I'm staining mouse lung tissue using the Envision+ kit 
> > from DAKO. I'm
> > > using a rabbit anti-PCNA from Santa Cruz biotech as the primary 
> > Ab. I incubate
> > > at RT for 1hr. Everything else is done as suggested by the kit 
> > (peroxidase> block, serum block, secondary Ab, chromogen, etc). I 
> > used the PCNA Ab at 1:50
> > > and got nothing. When I went to 1:25, I had a lot of backrgound 
> > and no stain
> > > in the nucleus whatsoever. I'm wondering if anyone has any 
> > suggestions on this
> > > stain, and also what is the best tissue from mouse to use as a 
> > positive> control. Thank you for your time.
> > > 
> > > Nidal E Muvarak
> > > Associate Research Specialist
> > > Department of Biomedical Engineering
> > > University of Wisconsin-Madison
> > > 1550 Engineering Dr.; Rm. 2158
> > > Madison, WI 53706-1609
> > > Lab: (608) 265-8921; Office: (608) 265-4205; 
> > > Home: (608) 256-7934; Cell: (608) 332-6068
> > > 
> > > 
> > > 
> > 
> >