RE: Quality of Fixation
|From:||Jenny Oblander (by way of Histonet)|
You might try Vimenten. If the tissue has not been fixed enough or fixed for
to long of a period, it will be negative. As far as showing degree of
fixation, I'm still looking for references. Hope this helps. Jenny
From: Charles W. Scouten, Ph.D. [mailto:email@example.com]
Sent: Thursday, March 06, 2003 9:27 AM
Subject: Quality of Fixation
I need some help from the experts.
Is there a standard measure of quality of fixation? A stain that shows
degree of fixation? I don't have a lot of equipment, and don't want to
spend a lot of money, but I have been challenged by a reviewer to show
that my perfusion methods (animal research) results in as good a
fixation as standard methods.
I can see it does, tissue quality is excellent for sectioning and
staining, but how do I quantify or show that? Age it 90 days and
report degree of crumbling? It is 4% formaldehyde fixation.
Charles W. Scouten, Ph.D.
5918 Evergreen Blvd.
St. Louis, MO 63134
Ph: 314 522 0300
FAX 314 522 0377
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