RE: Quality of Fixation

From:Jenny Oblander (by way of Histonet)

You might try Vimenten. If the tissue has not been fixed enough or fixed for
to long of a period, it will be negative. As far as showing degree of
fixation, I'm still looking for references. Hope this helps. Jenny

-----Original Message-----
From: Charles W. Scouten, Ph.D. []
Sent: Thursday, March 06, 2003 9:27 AM
Subject: Quality of Fixation

I need some help from the experts.

Is there a standard measure of quality of fixation?  A stain that shows
degree of fixation?  I don't have a lot of equipment, and don't want to
spend a lot of money, but I have been challenged by a reviewer to show
that my perfusion methods (animal research) results in as good a
fixation as standard methods.

I can see it does, tissue quality is excellent for sectioning and
staining,  but how do I quantify or show that?  Age it 90 days and
report degree of crumbling?  It is 4% formaldehyde fixation.


Charles W.  Scouten, Ph.D.
5918 Evergreen Blvd.
St. Louis, MO 63134
Ph: 314 522 0300
FAX  314 522 0377

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