Richard

We use the Santa Cruz rabbit Polyclonal PDGFRalpha Antibody (Cat Number
sc-338). We carry out microwave antigen retrieval using Target retrieval
solution (DAKO) on FFPE material. Overnight incubation at a dilution of
1/400. My control tissue is Glioma.

Cheers

Jim Manavis
Senior Hospital Scientist
Department of Neuropathology
Institute of Medical & Veterinary Science
Adelaide, SA, 5000
Australia
Phone: 61-08-8222-3668
FAX: 61-08-8222 3204
e.mail: jim.manavis@imvs.sa.gov.au
Disclaimer: Not this little black duck!


-----Original Message-----
From: Richard Cartun [mailto:Rcartun@harthosp.org]
Sent: Tuesday, 11 March 2003 10:58 PM
To: Histonet@pathology.swmed.edu
Subject: PDGFR - IHC


Is anyone doing IHC for "platelet-derived growth factor receptor"
(PDGFR)?  If so, can it be done on routinely processed, formalin-fixed
tissue?  Thank you.

Richard Cartun