RE: Counterstain for lacZ

From:"Montague, Donna C"

I like neutral red or very dilute saffarin o (like 0.1 % for 30 sec). Donna
University of Arkansas for Medical Sciences
Department of Physiology & Biophysics and
Center for Orthopaedic Research
4301 W. Markham St. # 505
Little Rock, AR 72205


-----Original Message-----
From: mrl0627@mail.ecu.edu [mailto:mrl0627@mail.ecu.edu] 
Sent: Wednesday, March 12, 2003 8:26 AM
To: histonet@pathology.swmed.edu
Subject: Counterstain for lacZ



Hello Histonetters!
I am looking for the best counterstain to display xgal staining present in mouse cochlea sections.  The area is very small, so I am afraid of overwhelming the blue staining present.  Have been advised to try Eosin for no more than 10 seconds on my dewaxed 5 micron sections; any thoughts/suggestions appreciated.  Thanks in advance.  Maureen @ East Carolina U.
								
			




<< Previous Message | Next Message >>