RE: Counterstain for lacZ

From:"Montague, Donna C"

I like neutral red or very dilute saffarin o (like 0.1 % for 30 sec). Donna
University of Arkansas for Medical Sciences
Department of Physiology & Biophysics and
Center for Orthopaedic Research
4301 W. Markham St. # 505
Little Rock, AR 72205

-----Original Message-----
From: [] 
Sent: Wednesday, March 12, 2003 8:26 AM
Subject: Counterstain for lacZ

Hello Histonetters!
I am looking for the best counterstain to display xgal staining present in mouse cochlea sections.  The area is very small, so I am afraid of overwhelming the blue staining present.  Have been advised to try Eosin for no more than 10 seconds on my dewaxed 5 micron sections; any thoughts/suggestions appreciated.  Thanks in advance.  Maureen @ East Carolina U.

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