From:Chris van der Loos

Dear Sylvia,
The problem of fading AEC reaction product sounds familiar to me. For some 
unknown reason I have some still beautiful red sections here stained in the 
mid 80-ies. However, all sections stained later start to fade after half a 
year. I still think that we have had a very special batch of AEC in the mid 
80-ies, that isn't produced ever again. For the best you protect your 
AEC-stained sections from direct (sun)light. The real precious ones (such 
as my course slides...) can be successfully stored at -20C.
Chris van der Loos
Dept. of Cardiovascular Pathology
Academic Medical Center
Amsterdam, The Netherlands

Sylvia Poulos wrote:
 >Date: 11 Mar 2003 08:16:02 -0600
 >From: Sylvia Poulos 
 >Subject: AEC
 >My lab's been using AEC as the color substrate of choice for several years.
 >The color was stable for at least 6 months until very recently. I'm seeing
 >that some staining lasts 6 months while others only last a onth or so. I
 >cover slip using glycerol gelatin. Any thoughts as to why the shift in
 >stability? I've tried using different batches of AEC with no change.
 >Those of you using DAB...can I use a water soluble mount with DAB? I'm not a
 >histo-buff...this is just a side part of my job so please pardon my obvious
 >lack of basic knowledge.
 >Sylvia Poulos
 >Animal Physiology Research Unit
 >Athens, GA 

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