The problem of fading AEC reaction product sounds familiar to me. For some
unknown reason I have some still beautiful red sections here stained in the
mid 80-ies. However, all sections stained later start to fade after half a
year. I still think that we have had a very special batch of AEC in the mid
80-ies, that isn't produced ever again. For the best you protect your
AEC-stained sections from direct (sun)light. The real precious ones (such
as my course slides...) can be successfully stored at -20C.
Chris van der Loos
Dept. of Cardiovascular Pathology
Academic Medical Center
Amsterdam, The Netherlands
Sylvia Poulos wrote:
>Date: 11 Mar 2003 08:16:02 -0600
>From: Sylvia Poulos
>My lab's been using AEC as the color substrate of choice for several years.
>The color was stable for at least 6 months until very recently. I'm seeing
>that some staining lasts 6 months while others only last a onth or so. I
>cover slip using glycerol gelatin. Any thoughts as to why the shift in
>stability? I've tried using different batches of AEC with no change.
>Those of you using DAB...can I use a water soluble mount with DAB? I'm not a
>histo-buff...this is just a side part of my job so please pardon my obvious
>lack of basic knowledge.
>Animal Physiology Research Unit
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