Quality of Fixation

From:"Charles W. Scouten, Ph.D."

I need some help from the experts.

Is there a standard measure of quality of fixation?  A stain that shows
degree of fixation?  I don't have a lot of equipment, and don't want to
spend a lot of money, but I have been challenged by a reviewer to show
that my perfusion methods (animal research) results in as good a
fixation as standard methods.  

I can see it does, tissue quality is excellent for sectioning and
staining,  but how do I quantify or show that?  Age it 90 days and
report degree of crumbling?  It is 4% formaldehyde fixation.


Charles W.  Scouten, Ph.D.
5918 Evergreen Blvd.
St. Louis, MO 63134
Ph: 314 522 0300  
FAX  314 522 0377

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