IF Staining on thick free floating sections
I was wondering if any of you out there have any recommendations for staining 100 micron free floating sections. The sections that I am working with are mouse cerebellum. These sections will be cut on a vibratome. The sections are embedded in a 2% Agarose Solution. I will be doing various Direct Immunofluorescent Stains on them. Any recommendations or protocols would be appreciated.
Thanks in advance,
University of Iowa Health Care System
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