IF Staining on thick free floating sections


Fellow histonetters:

I was wondering if any of you out there have any recommendations for staining 100 micron free floating sections.  The sections that I am working with are mouse cerebellum.  These sections will be cut on a vibratome.  The sections are embedded in a 2% Agarose Solution.  I will be doing various Direct Immunofluorescent Stains on them.  Any recommendations or protocols would be appreciated.

Thanks in advance,

Steven Westra
University of Iowa Health Care System
(319) 356-1090
(319) 335-8889

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