GFP in mouse tissue
Hi,
I have to visualize GFP tagged cells (EGFP and dsRED 2 from Clontech) in mouse
tissue (lung, liver, brain and maybe bone). I heard very different things
about the ethanol stability of GFP and the feasibility of different protocols
for cryosections or parraffin. Does anybody has experience with this problem
or maybe even a protocol that works ?
Any help is very much appreciated.Thank you very much.
Yours sincerely
Martin Weber
Martin Weber M.D.
The Scripps Research Institute, MEM 175
10550 North Torrey Pines Road
La Jolla, 92037 C.A., U.S.A.
phone: 858-784-8612 (w) or 858-452-0544 (h)
FAX: 858-784-2030
E-Mail: maweber@scripps.edu
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