In addition, Applied Immunohistochemistry & Molecular Morphology 11(1): 51-55, 2003 has an article regarding c-kit expression in pulmonary small cell Ca which contrasts the Dako and Santa Cruz antibody with and without HIER pre-treatment.  They don't mention any non-specific cross reactivity with fibroblast and myofibroblasts, only with normal pancreatic tissue.  They used the Ventana ES immunostainer.

Regards,

Teri Johnson
Managing Director Histology Facility
Stowers Institute for Medical Research
1000 E. 50th St.
Kansas City, Missouri  64110
tjj@stowers-institute.org



-----Original Message-----
From: Carrie Kyle-Byrne [mailto:ckbyrne@exelixis.com]
Sent: Thursday, March 13, 2003 3:18 PM
To: 'HistoNet@Pathology.swmed.edu'
Subject: FWD'ing a post for Ronnie Houston RE c-kit


One of our pathologists has shared some information he was given by a doctor
at AFIP. I have never seen this before, but was wondering if anyone else
has.

They claim that the c-Kit antibody from Dako(A4502)gives "widespread
positivty of fibroblasts and myofibroblasts, suggesting non-specific cross
reactivity with this particular antibody". However, they did not experience
this phenomenon with the c-kit from Santa Cruz Biotechnology (sc-168). Dako
are not aware of any issues with their antibody.

Both of these are polyclonals.

Appreciate your input
Thanks
Ronnie 

Ronnie Houston
Regional Histology Operations Manager
Bon Secours HealthPartners Laboratories
5801 Bremo Road
Richmond, VA 23226
(804) 287 7972
ronnie_houston@bshsi.com