Tungsten Carbide knife - embedded in PMMA for microtomed sections, but
teeth must be fixed, PMMA can be removed BEFORE staining - IHC would
require stringent antigen retrieval - Neil Hand has excellent retrieval
method published in J Histotechnology in 1999 or so. 

For frozen sections, more difficult to get a good section without
Instrumedics Cryojane tape transfer plus you need to use a tungsten carbide
blade, d profile

You may have results using EDTA decalcification and cut in cryostat.  This
can even be done on fresh unfixed or unfixed bone/teeth, fix frozen
sections before staining. 

Check out J of Histochemistry Cytochemistry, a recent publication did a
similar method using PLP fixation, etc. 

Also Mori S et al J Histochem Cytochem, 36:111-114, 1988 did EDTA on
unfixed fresh bone for cell surface markers. 

Not an easy project!
 



Gayle Callis
MT,HT,HTL(ASCP)
Research Histopathology Supervisor
Veterinary Molecular Biology - Marsh Lab
Montana State University - Bozeman
S. 19th and Lincoln St
Bozeman MT 59717-3610

406 994-6367 (lab with voice mail)
406 994-4303 (FAX)

email: gcallis@montana.edu