Hi Everyone

 I'm cutting 40 um paraffin sections, human brain, so the sections is very big. The problem is, that some sections (to much) is coming loose from the slides when I rehydrate. I coated the slides with 4,5% gelatine and 4,0% potassium chromic sulphate.  I have tried silane slides but the gelatine/ChromeAlun seems to be the best for now.

If anyone has a suggestion to overcome the problem with loose section, I would like to here from you.

Thanks

Sus Sørensen
Research Lab Stereology & Neuroscience
Denmark
susr@city.dk