40 um paraffin sections coming loose from the slides


Hi Everyone

 I'm cutting 40 um paraffin sections, human brain, so the sections is very big. The problem is, that some sections (to much) is coming loose from the slides when I rehydrate. I coated the slides with 4,5% gelatine and 4,0% potassium chromic sulphate.  I have tried silane slides but the gelatine/ChromeAlun seems to be the best for now.

If anyone has a suggestion to overcome the problem with loose section, I would like to here from you.


Sus Sørensen
Research Lab Stereology & Neuroscience


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