sectioning of paraffin-embedded tissue

From:sebres@comcast.net

I hope you folks will forgive a very naive question:  I have years of
experience sectioning fresh frozen tissue in a cryostat-minotome, but
suddenly need to section paraffin-embedded (formalin-fixed) material for the
first time.  Can I do this in my cryostat?  Does the temperature matter?
How would I affix the block to the chuck, just melt it on?   Thank you very
kindly for any advice!  Susan Bachus





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