Sorry, more comments

This method supposedly works best on decalcified frozen sections or
celloidin embedded bone, freshly cut,  and there can be variability if the
pH is not optimal or lots of thionin can vary.  Seems like a tedious method  

 
Gayle Callis
MT,HT,HTL(ASCP)
Histopathology Supervisor
Veterinary Molecular Biology - Marsh Lab
Montana State University - Bozeman
19th and Lincoln St
Bozeman MT 59717-3610

406 994-6367
406 994-4303 (FAX)