frozen sections and Purkinje fibers



I have received from a researcher a number of frozen section slides of
primate heart.  The slides were fixed in 4% paraformaldehyde and are now
air-dried.  They want the Purkinje fibers demonstrated.

1)	Any suggestions?

2)	If I stain these frozens with Masson's Trichrome, do I need to mordant in

3)	Should I start with Alcohol and then hydrate or go directly to water or
directly into a stain?

Sorry if this last question sounds elementary, but we don't do frozens in
this lab. Thanks for any help.

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