factor XIIIa

From:Jeff Silverman

I have worked extensively with factor XIIIa and am very interested in it as
a marker for stromal histiocytes in fibrohistiocytic and fibrovascular
tumors. I get the best results with FFPE tissues  with a thirty minute
enzymatic digestion at  37 deg C using the prebuffered trypsin tablets from
Sigma. HIER is way too harsh and way overkill, causing often intense
background staining. I use Calbiochem rabbit anti-human factor XIIIa 1:400
in PBS with 1% bovine serum albumin. If its not getting too personal, I'd be
interested to know what you are using it for. Hope this helps.
Jeff Silverman HT HTL QIHC (ASCP)
Southside Hospital
Bay SHore, NY USA 11937

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