Re: need c-fos monoclonal antibody

From:

Kathleen Spencer

Re: need c-fos monoclonal antibody I use two c-fos ab's that work quite well, but they are polyclonal. Does it have to be monoclonal?

I would call the company you purchased them from for technical assistance. They supposedly have people testing these ab's.

Kathleen Spencer, H.T.
LCM Supervisor
University of Tennessee
901.448.3720

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From: Mary-Ann S Crissey <Mary-Ann.Crissey@bms.com>
To: Histonet <histonet@pathology.swmed.edu>
Subject: need c-fos monoclonal antibody
Date: Tue, Mar 26, 2002, 10:02 AM

I am looking for a monoclonal c-fos antibody that works for immunohistochemistry staining. We are working with perfusion fixed free floating rat brain sections and want to do double staining. I've already tried 3 different monoclonals that failed to give nuclear staining. Anybody have any experience with one that works?

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