Re: extended time in NBF
If they want to do apoptosis by IHC methods it is probably not a good
idea to leave the samples in formalin for more than a couple of days, because
the proteins will be crosslinked making access difficult. Antigen
retriveal methods to break the links can be used but it is always easier
to start with accessibility. As far as drug characterization, it
depends on what methods they will be using to do this.
A grad student is wondering how long tumor samples can be left in !0%
neutral buffered formalin for future apoptosis and drug characterization
(she did not elaborate). Is several weeks too long?
I do not do either procedure. You can email her : Victoria
Thanks for your help.
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