Just a thought....dirty slides (the biggest culprit being fingerprints) can create the problems like this.

Barb Davies
Memorial hospital
Colorado Springs, CO




"Sebree Linda A." 


"Sebree Linda A."  on 03/18/2002 02:54:34 PM

To:   'Histonet' 
cc:

Subject:  bone marrow biopsy tissue adherence


Hello colleagues,

I need help in solving a sporadic but ongoing problem.  We process our bone
marrow biopsies using B5 fixative and RDO decal solution.  Tissues are cut
onto "Plus" slides for IHC staining, air-dried and baked @ 60 degrees C for
at least 30 minutes or if time is critical, microwaved for 2 minutes.  When
time is less of an issue, we dry our slides at room temperature, 37 degrees
C or 60 degrees C overnight, depending on what we think will result in the
best tissue adherence.

Here in lies the problem:  no matter how well or difficult the tissue is to
cut or how the tissue has been dried/baked we experience tissue loss on some
cases.  There is no rhyme or reason as to which cases will fall off; it
happens on a case-by-case basis.

Any suggestions in terms of preparation of slides, drying, baking, etc.
would be appreciated.  We do not have any authorization to change the
fixation, decaling or processing procedures.

Thanks in advance,

Linda A. Sebree, HT(ASCP)
University of Wisconsin Hospital & Clinics
IHC/ISH Laboratory
A4/204-2472
600 Highland Ave.
Madison, WI 53792-2472
(608)265-6596
FAX: (608)262-7174