Re: Returned mail: User unknown
Dear Histonet,
1. I have problems staining tendon with Masson Trichrome. It seems
like parts of the fibers were dissolved by the method. I've stained
same set of tendons with Alcian Blue, pH 2.5, and there was no obvious
change. So I'd assume that acetic acid wasn't at fault. I'd think
the phosphomolybdic acid was the culprit. I have repeated the
procedure several times. When I stained femoral head sections with
MT, it worked nicely. Please help. Is there any substitute for
phosphomolybdic acid? Tissues were fixed in Bouin's solution. Since
the tendons are quite thick, 5-8mm, they were fixed for 3 days. The
excess picric acid was removed after rehydration of the tissues prior
to hematoxylin step, not prior to processing.
2. What will happen if I forgot to shake the tissues for the first 12
hours of fixation? (Bouin's fixative solution was used.)
Thank you in advance
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