Re: Frozen Section Sllides
I always store frozen sections slides at -80C after they are air dryed unfixed
and individually wrapped in foil in a zip lock bag with some dessicant in the
bottom. Then when I am ready to stain I take a jar of cold acetone to the
freezer site and unwrap each slide individually and put them directly into the
acetone. If I am going to use all the slides in the zip lock bag, I bring the
entire bag out of the freezer and let it sit at room temp without opening it for
20 min. or so to climitize. When I open the bag after this the slides are dry
and no longer cold. I then fix the slides with various fixatives depending on
what I am doing. I recommend that you send the unfixed slides on dry ice and
let the people you are sending to fix just before they stain.
Cynthia Stone wrote:
> I'm testing some different ways to prepare unstainned frozen section slides
> to be sent out. I've tried putting them in a slide box inside a bitran bag
> and leaving them in the -80 freezer for a couple of days to pretend they
> were in transit. Then I removed the slides and fixed one in acetone and it
> stained fine. Clear, sharp stain. One fixed in Rapid Fix didn't stain very
> sharp. Could it be some moisture on the slide or is it more difficult to
> dissolve the OCT on the slide the longer the slides are stored before
> stainning? Is any one fixative better to use? Acetone may be a little more
> forgiving when it comes to moisture, but what about some type of alcohol?
> How do other send and fix unstainned frozen slides for H&E , immuno's, and
> In Situ ? Thanks for any suggestions, Cindy Stone.
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