Re: Fluoro Question

From:"John C. Dennis"


Erythrocytes autofluoresce.  They're pretty easy to ID by morphology so
you shouldn't have too much problem keeping track of them on your tissue

Yours seeing Red,

John Carroll Dennis
Anatomy, Physiology, and Pharmacology
109 Greene Hall
Auburn University, AL  36849

On Tue, 26 Mar 2002, Tarpley, John wrote:

> I haven't done a lot of work with fluorescence IHC, but we now need to do
> some CD31 staining and use either an Alexa Fluor 594 or Texas Red conjugated
> secondary antibody for detection. We get very nice CD31 staining, but we
> also get rather strong staining of RBCs within and outside of blood vessels.
> I've tried reducing the concentration of the secodaries, but the staining
> still persists. Slides done with buffer control for the CD31 are clean with
> the exception of the RBC staining. Both secondaries tested so far are from
> Molecular Probes. I think this is a fine company and their technical
> department is trying to give us assistance but so far we have not solved the
> problem. This afternoon we will try a run using a secondary from another
> company just to see what happens. Any suggestions for solving this problem
> will be most welcome.
> John E. Tarpley 5-1-A
> Associate Scientist
> Amgen Inc.
> One Amgen Center Drive
> Thousand Oaks, CA 91320

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