RE: processing brain sections
I can give you a protocol for mouse brain run using a Shandon citadel.
Protocol should vary depending on size of tissue and type of processor.
From: Anacko, Nancy L. [mailto:NAnacko@Lifespan.org]
Sent: Wednesday, March 20, 2002 6:38 AM
To: 'Server, Histonet'
Subject: processing brain sections
Can someone help me ?? How should my processor be set up to process brain ??
I use 2 cont. 10% neutral buffered formalin, 1 cont. 70% alc., 1 cont. 80%
alc., 2 cont. 95% alc., 2 cont.100% alc., 2 cont. xylene and of course 2 wax
chambers. I couldn't tell you the times in each because the screen on my
shandon is burnt out, I'm new to the dept. and can't find our procedure.
Soooo if anyone could send me their procedure....it would be appreciated.
Maybe I could get my processor fixed or a new one (hi,diane if you're
reading this :))
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