RE: What are vibratome sections used for?

From:"Haines, Ashley N."

Thanks for calling us courageous!  We regularly use vibratome sections for
IHC.  We esp. use it for detecting tracer in brain tissue, again with DAB

In particular we use vibratome sections instead of frozen sections in an
attempt to preserve cell size.  We have a lot of concerns re changes in cell
size with cryoprotection and freezing.
Anyone have any insight in this area?  I think its partly philosophical,

It will work well on tissues other than brain, but they must be dense and
very well fixed...

> Just wondering, I've seen vibratomes for years but never really understood
> what people are using them for. Anyone?
> Tim Morken
> CDC, Atlanta

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