Can you really get decent EM from frozen sections embedded in plastic? What sort of cryoprotection do you do?

Sidney. 

Paul Klosen  wrote:

>I for my part don't like the vibratome. Actually, I get criminal urges if
>someone (my boss ...) suggest I should use it. If I have to do
>pre-embedding EM I use well cryoprotected tissue frozen sections. They >work just as well, but are less bothersome to produce (1 frozen section = >one turn of the handle, 1 vibratome section = about 1 minute and lots of nerves)
>-) O
>===============================oOo==(_)==OOo=============================
>Paul Klosen, PhD
>CNRS UMR 7518 Neurobiologie des Fonctions Rythmiques et Saisonnieres
>Universite Louis Pasteur  12, rue de l'Universite
>F-67000 Strasbourg, FRANCE
>Tel. 03.90.24.05.01  Fax. 03.90.24.05.28
>========================klosen@neurochem.u-strasbg.fr=====================
-- 




__________________________________________________________________
Your favorite stores, helpful shopping tools and great gift ideas. Experience the convenience of buying online with Shop@Netscape! http://shopnow.netscape.com/

Get your own FREE, personal Netscape Mail account today at http://webmail.netscape.com/