RE: Nile Blue Sulfate vital staining
Look up S. Kimura and K. Shiota. Sequential changes of programmed cell death in developing fetal mouse limbs and its possible roles in limb morphogenesis. Journal of Morphology vol 229 pages 337-346 (1996).
They used Nile blue to stain apoptotic nuclei, which are seen in cells that died by apoptosis.
Ralph Marcucio wrote:
>I have been trying to detect cell death in whole embryos using Nile Blue
>sulfate, and to date, I have had no luck, even in my very positive controls.
>Does anyone have a protocol for using Nile Blue Sulfate to for vital
>Thanks in advance,
>Ralph Marcucio, PhD
>Department of Orthopaedic Surgery
>University of California at San Francisco
>San Francisco, Ca 94143
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