Tammy,
Never done cow, have done cat smears. Fix in 95% Ethanol (if not too late,
otherwise Para should be OK) and fix for about 20 minutes. Then run down to
water, stain H&E, not long in Hematoxylin, 1 minute should do it. Best of
luck! Gotta love research ;)

Mary Georger
Center for Cardiovascular Research
University of Rochester Medical Center
KMRB Room 2-9816
601 Elmwood Avenue.
PO Box 679
Rochester, New York  14642
585-273-1548

> ----------
> From: 	Tammy Bailey
> Sent: 	Tuesday, March 19, 2002 1:20 PM
> To: 	histonet@pathology.swmed.edu
> Subject: 	I'm lost
> 
> Does anyone have some suggestions for a small prob I have. A few minutes
> ago, a researcher dropped by with a few slides of fresh cow retina
> smears(not sections)he wants a quick stain showing the cellular
> structure...mostly cones...and membranes...Of course I've never done these
> smears before....I'm sure I'm going to have to fix the slides before
> staining....any suggestions?? This is a first time try for him(and I must
> say me too). I was considering a tol. blue stain...or maybe a good old
> H&E....He is really wanting to look at the retinal cellular structure. Any
> suggestions please hurry..this fresh cow smear is getting smelly....I
> think
> I'll move it right away into a 2% para....the things we do...geezz...
> 
> tammy
> 
> 
> Tammy Bailey	      		        / /
> Histology Analysis Lab, VSRC               / /\
> 310 Worrell Building				/ /\ \
> University of Ala. at Birmingham         / /  \ \
> Phone #:  (205) 934-3074                / /    \ \
> Fax #:  (205) 934-5725			  ______   ((0))
> email: tbailey@uab.edu                 |__|    / /
> Cell #:205-410-8029                     ||    / /
>                                      --------------
>                                    ------------------
> 
> 
>