OK BonesSlides,

Here's

Goldner's Stain:  p337 in Gray (below)

Soln 0:  Weigert, 1904:  (A)HOH 95 + FeCl3 0.6g; (B) 1% hematoxylin in 95%
EtOH.  Make Soln 0 with equal parts (A) and (B)
Soln A:  HOH 100 + .07g Ponceau 2R + acid fuchsin 0.03 + HOAc 0.2ml
Soln B:  1% HoAc
Soln C:  HOH 100 + phosphomolybdic 4 + Orange G 2
Soln D:  HOH 100 + Light Green 0.2g + HOAc 0.2ml

Method:
	Soln 0 'till colored then tapwater to blue
	Soln A:  5min and into Soln B
	Wash
	Soln C: 'til collagen colored and into Soln B
	Rinse
	Soln D:  5min and into Soln B 5min and blot
	Absolute EtOH for minimum time
	Xylene to Balsam

Result:  general cytoplasm red, RBC's orange, collagen green

That's what the book says.  You must REALLY want this one.

Regards,

Fred Monson

Frederick C. Monson, PhD   
Center for Advanced Scientific Imaging  
West Chester University  
West Chester, Pennsylvania, USA, 19383
610-738-0437
fmonson@wcupa.edu


P.S.  How have you survived without the following book?
	

BOOK #:     202473      ISBN #:      0-88275-247-2
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Melbourne, Florida U.S.A. 32902-9542
Phone (321) 724-9542 ·  Fax (321) 951-3671

Suffering from old-age and hockey fever at the same time is HELL!!!  Go
North America!!!!


> ----------
> From: 	Boneslides@aol.com
> Sent: 	Wednesday, March 27, 2002 2:30 PM
> To: 	histonet@pathology.swmed.edu
> Subject: 	Goldner's Trichrome
> 
> Many, many moons ago, I used to do a Goldner's trichrome on PMMA sections.
> I 
> have a researcher asking for that stain, and, of course, I can't find a 
> procedure anywhere.  Does anyone have a protocol that they can share?  I 
> would greatly appreciate any help!!
> 
> Thanks,
> 
> Diane Mahovlic, HT(ASCP)
> The Cleveland Clinic Foundation
> Cleveland, Ohio   
> 
>