From:Tamara Howard

Jenny -

GFP (Green Fluorescent Protein) may make it through paraffin processing
and still fluoresce - it seems to depend on the construct itself - so I'd
give it a whirl and see if you can still get glowing whatever it is that
is expressing. If your construct does not like being dehydrated and
heated, there are several antibodies to GFP available, but the same caveat
seems to apply - depends on the construct. Some people have had great
success with this; others have headaches & nightmares :)

The bottom line is, does your researcher really need paraffin sections? No
access to a nice confocal or multi-photon system on which you could do
whole mount or vibratome slices? Life would be much easier!

Good luck.


 Tamara Howard
 Department of Cell Biology and Physiology
 University of New Mexico - Health Sciences Center
 Albuquerque, NM 87131

<< Previous Message | Next Message >>