Answers below.

Fred Monson

> ----------
> From: 	Kimberly L Merriam
> Sent: 	Wednesday, March 20, 2002 12:19 PM
> To: 	Histonet
> Subject: 	Evans Blue Redux
> 
> <>
> Hi,
> 
> Thanks to everyone that sent me information on Evans Blue
> dye.  I have some more questions regarding Evans Blue.
> 
> 1. If I inject Evans Blue into an animal (rat or mouse),
> will I be able to see it microscopically?
> 
If there is extravasation, the answer is yes.  Otherwise, if injected i.v.,
the color will remain in the vascular space.  PDF of paper under separate
cover should give good information.

> 2. How long after injection should I sac the animal?
> 
What's the half-life of albumen?

> 3. What percentage of dye should I use?
> 
0.6ml of 1% EB in tail vein.(appropriate PDF attached separately)

> Any information would be appreciated.
> 
> Thanks,
> Kim Merriam
> TKT
> Cambridge, MA
> 
>