Ginny,
You may want to consider which fixative you will need since this is going to
EM.  Some EM people are not fond of getting the tissue in 10%NBF.  The
method Ginny recommends is great and ideal for handling the transport.  Pam
Marcum

> -----Original Message-----
> From: Jacqueline D. Garfield [mailto:JGarfield@lifecell.com]
> Sent: Tuesday, March 19, 2002 11:08 AM
> To: 'Einfalt, Ginny'; 'histonet@pathology.swmed.edu'
> Subject: RE: EM
>
>
> Dear Ginny,
>
> I previoiusly worked in a hospital EM lab where kidney biopsies (bx.) were
> our routine work.  We served many outside hospitals.  If the
> hospital who is
> sending us the bx., could get us the sample in less than 2 hours from
> procurement, then we would ask them to carefully wrap the fresh tissue in
> gauze saturated with saline.  Put the gauze in a specimen cup
> with a little
> extra saline to keep it moist.  Then pack the specimen cup on ice
> to keep it
> cold, and immediately drive it over.
> However, if you are more than 2 hours away from the EM lab, or if
> it will be
> recieved after hours, then it would be best to request vials of fixative
> from them.  This is actually the best way to prevent the tissue
> from drying
> out and preserve the ultrastructure.
>
> I hope this is helpful.
> Good Luck,
>
> Jackie Garfield
> LifeCell Corporation
> Branchburg, NJ
>
> > -----Original Message-----
> > From:	Einfalt, Ginny [SMTP:GEINFALT@virtua.org]
> > Sent:	Tuesday, March 19, 2002 9:49 AM
> > To:	'histonet@pathology.swmed.edu'
> > Subject:	EM
> >
> > We currently send out our kidney biopsies, but I am interested in
> > collection
> > methods, how long the specimen is viable, etc.  Any EM techs
> out there, I
> > need some expert imput!
> >
> > Thanks,
> > Ginny Einfalt  HT/HTL(ASCP)
> > Corporate Histology Manager, Virtua Health
> > 856-325-3144
> > 856-325-3317 Fax
> > geinfalt@virtua.org
> >
>
>