You may want to consider which fixative you will need since this is going to
EM. Some EM people are not fond of getting the tissue in 10%NBF. The
method Ginny recommends is great and ideal for handling the transport. Pam
> -----Original Message-----
> From: Jacqueline D. Garfield [mailto:JGarfield@lifecell.com]
> Sent: Tuesday, March 19, 2002 11:08 AM
> To: 'Einfalt, Ginny'; 'email@example.com'
> Subject: RE: EM
> Dear Ginny,
> I previoiusly worked in a hospital EM lab where kidney biopsies (bx.) were
> our routine work. We served many outside hospitals. If the
> hospital who is
> sending us the bx., could get us the sample in less than 2 hours from
> procurement, then we would ask them to carefully wrap the fresh tissue in
> gauze saturated with saline. Put the gauze in a specimen cup
> with a little
> extra saline to keep it moist. Then pack the specimen cup on ice
> to keep it
> cold, and immediately drive it over.
> However, if you are more than 2 hours away from the EM lab, or if
> it will be
> recieved after hours, then it would be best to request vials of fixative
> from them. This is actually the best way to prevent the tissue
> from drying
> out and preserve the ultrastructure.
> I hope this is helpful.
> Good Luck,
> Jackie Garfield
> LifeCell Corporation
> Branchburg, NJ
> > -----Original Message-----
> > From: Einfalt, Ginny [SMTP:GEINFALT@virtua.org]
> > Sent: Tuesday, March 19, 2002 9:49 AM
> > To: 'firstname.lastname@example.org'
> > Subject: EM
> > We currently send out our kidney biopsies, but I am interested in
> > collection
> > methods, how long the specimen is viable, etc. Any EM techs
> out there, I
> > need some expert imput!
> > Thanks,
> > Ginny Einfalt HT/HTL(ASCP)
> > Corporate Histology Manager, Virtua Health
> > 856-325-3144
> > 856-325-3317 Fax
> > email@example.com
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