RE: Daily Digest
Regarding the Leica 2125 microtomes - we have 3 of these and are hoping to
get 2 more. We demo'd quite a few different ones and definitely liked these
best.
-----Original Message-----
From: HistoNet Server [mailto:histonet@pathology.swmed.edu]
Sent: Thursday, March 28, 2002 1:06 AM
To: HistoNet Server
Subject: Daily Digest
----------------------------------------------------------------------
Date: 27 Mar 2002 00:02:13 -0600
From: "Mcleod, H, Heather"
Subject: fatty acid synthase
Dear Histonetters
Please HELP!! I am trying to purchase an antibody to fatty acid
synthase but have been unable to find a supplier. A reference I
have, used an antibody supplied by IBL Japan. Does anyone know if
there are agents for this company in Europe or even better South
Africa?
Thankyou
Heather
----------------------------------------------------------------------
Date: 27 Mar 2002 00:20:50 -0600
From: Scott Sauerbrunn
Subject: Scott Sauerbrunn/ops/diag/sial is out of the office.
I will be out of the office starting 03/25/2002 and will
not return until 04/01/2002.
I will respond to your message when I return.
----------------------------------------------------------------------
Date: 27 Mar 2002 03:16:09 -0600
From: "Steve Machin UK"
Subject: MSDS and Safety Assessments on a new lab site
MSDS on many lab chemicals can be found on this site
http://www.merckeurolab.ltd.uk/template.asp?file=a1MSDS.htm
A new biomedical science site has been set up where users submit their own
protocols to be downloaded by other users.
http://www.greyzone.freeserve.co.uk
I have put our labs COSHH assessments (safety assessments for hazardous
substances) on it, about 600 substances and prepared reagents in total in MS
Word format. These are linked by two MS Excel index files. The zipped file
expands into about 17Mb. If you download it please ignore the file called
"a_master_index.doc"; I should not have included it.
Best Wishes
Steve Machin UK
- ---------------------------------
Do You Yahoo!?
Get personalised at My Yahoo!.
******************* NOTE *******************
There may be important message content
contained in the following MIME Information.
********************************************
- ------------------ MIME Information follows ------------------
- --Boundary_(ID_cycDe5Eq+efbPDbAq0nSIA)
Content-type: text/plain; charset=iso-8859-1
Content-transfer-encoding: 7BIT
<<<<<< See above "Message Body" >>>>>>
- --Boundary_(ID_cycDe5Eq+efbPDbAq0nSIA)
Content-type: text/html; charset=iso-8859-1
Content-transfer-encoding: 7BIT
MSDS on many lab chemicals can be found on this site http://ww
w.merckeurolab.ltd.uk/template.asp?file=a1MSDS.htm
A new biomedical science site has been set up where users submit their
own
protocols to be downloaded by other users. http://www.greyzone.freeserve.co
.uk
I have put our labs COSHH assessments (safety assessments for
hazardous substances) on it, about 600 substances and prepared reagents in
total in MS Word format. These are linked by two MS Excel index
files. The zipped file expands into about 17Mb. If you
download it please ignore the file called "a_master_index.doc"; I
should
not have included it.
Best Wishes
Steve Machin UK
Do You Yahoo!?
Get personalised at My Yahoo!.
- --Boundary_(ID_cycDe5Eq+efbPDbAq0nSIA)--
----------------------------------------------------------------------
Date: 27 Mar 2002 04:31:37 -0600
From: Lee & Peggy Wenk
Subject: Re: cork cutting boards
I don't think there is an "official" regulation that forbids the use of cork
boards.
However, for us, switching from cork boards to the "plastic" boards was a
matter of trying to reduce the levels of formalin vapors to which our PAs
and Residents are exposed, and to ensure their safety for things
biohazardous.
Cork is porous, so it absorbs the formalin fluid. This fluid then
out-gasses.
Cork absorbs other fluids, like body/cell fluid, which if fresh, is
biohazardous. A quick scrub down with diluted bleach could not guarantee
that all the micro-organisms inside the board were killed.
So, in a round about way, switching to the "plastic" boards was influenced
by the following standards:
- - Chemical Hygiene Standard (reduce exposure to all chemicals)
- - Formaldehyde Standards (reduce exposure to formaldehyde)
- - Blood borne Pathogen Standard (reduce exposure to pathogens)
That was our reasoning. Hope it helps you in your decision.
Peggy A. Wenk, HTL(ASCP)SLS
William Beaumont Hospital
Royal Oak, MI 48073
- ----- Original Message -----
From: "Carmen Badley"
To:
Sent: Tuesday, March 26, 2002 11:39 AM
Subject: cork cutting boards
Does anyone know if there is a CAP or JCACHO
regulation forbidding Cork cutting boards used for
grossing? I was told sometime in the past that there
was. Please let me know. Thank you.
Carmen Badley; HT Sr. Tech
St. Joseph Mercy Oakland, MI
__________________________________________________
Do You Yahoo!?
Yahoo! Movies - coverage of the 74th Academy Awards(r)
http://movies.yahoo.com/
----------------------------------------------------------------------
Date: 27 Mar 2002 06:31:54 -0600
From: Dudecare@aol.com
Subject: Re: Grocott Methenamine Silver
Yes, if the silver is way overdone, and there is no replacement tissue, what
do you do??
----------------------------------------------------------------------
Date: 27 Mar 2002 07:32:12 -0600
From: Walzer Susan
Subject: Digital Camera
My pathologist is in the market for a new camera, is there a good one that
can attach to a microscope? (digital) His old one was 35mm. Thanks!
----------------------------------------------------------------------
Date: 27 Mar 2002 07:32:29 -0600
From: Walzer Susan
Subject: Leica 2125 Microtome
Can anyone tell me if this is a good mictrotome? Thanks!
----------------------------------------------------------------------
Date: 27 Mar 2002 08:16:36 -0600
From: "Milford, Kevin"
Subject: Xylene Neutralizing Pad
Does anyone know if there is any type of xylene neutralizing pad on the
market.
Kevin Milford
Schering-Plough Research Institute
Lafayette NJ 07848
phone (973) 940-4309
fax (973) 940-4120
***************************************************************
This message and any attachments is solely for the intended recipient. If
you are not the intended recipient, disclosure, copying, use, or
distribution of the information included in this message is prohibited --
please immediately and permanently delete this message.
----------------------------------------------------------------------
Date: 27 Mar 2002 08:32:56 -0600
From: DGoodwin@CHSNJ.org
Subject: Ab Source
Can anyone suggest a source for B72-3 (Tumor-associated glycoprotein) and
Ber-EP4 (epithelial specific antigen or ESA)?
Many thanks,
Diana Goodwin, HT
Trenton, NJ
This electronic transmission and any attachments hereto contains material
that is private and confidential in nature and may be protected by law. It
is for the intended recipient only. Any other disclosures are unintended.
If you have received this transmission, or a copy thereof, in error you are
instructed to immediately and permanently delete this transmission and any
attachments, and to notify the sender of such error by telephone at (609)
394-4000. Thank you for your
cooperation and assistance
----------------------------------------------------------------------
Date: 27 Mar 2002 08:46:01 -0600
From: Dimitri Scholz
Subject: Re: Digital Camera
Leica has developed very good, easy but rather expensive digital
cameras with the special microscopical software.
We have got a positive experience with the ordinary Canon D30 (for
Europe) digital photocamera with about 2.7 Mio pixels for about 5000
DM (last year).
Good luck
dimitri
- --
_________________________________________
Dr. Dimitri Scholz
Max-Planck-Institute
Dept. of Exp. Cardiology
Beneke-Str.2
D-61231 Bad Nauheim
Germany
voice ++49 6032 705-403
Fax ++49 6032 705-419
Email d.scholz@kerckhoff.mpg.de
----------------------------------------------------------------------
Date: 27 Mar 2002 08:51:36 -0600
From: =?UNKNOWN?Q?Agust=EDn?= Venzano
Subject: E 600 Nikon microscope
Dimaano, Nena replied:
Please go to Nikon website and type the catalogue that you are looking for
and you will be able to download.
thanks,
nena
NDimaano@howost.com
- -----Original Message-----
From: =?UNKNOWN?Q?Agust=EDn?= Venzano [mailto:avenzano@cicv.inta.gov.ar]
Sent: Monday, March 25, 2002 1:34 PM
To: HistoNet Server
Subject: Nikon microscope
Dear netters: There is a Nikon microscope model E 600 with an attached
FDX-35 microcamera in one lab of our Research Center. We accidentally lost
the user's catalogue, so now we're having some problems to take photographs.
Is there anyone out there who can send us an attachment with the catalogue?
Thank you in advance
Agustin Venzano
Lab of Veterinary Histopathology
INTA Castelar, Argentina
Dear Histonetters: I've already visited Nikon website, but I couldn't find
any information on the FDX-35 microcamera attached to the top of the E 600
Nikon microscope, nor could I get the user's catalogue. Does anyone out
there have such models of Nikon microscope and microcamera in his lab? If
you can help me, please submit the catalogues as an attachment.
Thank you
Agustin Venzano
Lab of Veterinary Histopathology
INTA Castelar, Argentina
----------------------------------------------------------------------
Date: 27 Mar 2002 08:57:41 -0600
From: "Monson, Frederick C."
Subject: FW: Digital Camera
> Easiest way is to get a Nikon Coolpix 995 Photomicrography Kit from your
> local Nikon microscope dealer. Comes with everything you need to attach
> it directly to the microscope. You might want to ask about a small
> monitor to go with it. Can't beat the monitor for teaching and
> collaborating. The Coolpix 995 will do dual duty on and off the
> microscope. Last price I got was in the vicinity of $1,500.00. NOTE:
> The Coolpix 995 is no longer in production, and the price on the street
> has really dropped. Purchasing it(3.4 MegaPixels) or the Coolpix 5000
> (5.0 MegaPixels) separately and then getting the appropriate couplers can
> be more trouble, but the total price can be much lower.
>
> Coolpix 995 site for photomicrography (note the product number (82055):
> http://www.nikonusa.com/usa_product/product.jsp?cat=5&grp=26&productNr=820
> 55
>
> Coolpix 5000 site for photomicrography (note the product number
> (82060):http://www.nikonusa.com/usa_product/product.jsp?cat=5&grp=26&produ
> ctNr=82060
>
> These sites explain the accessory configuration for both cameras and will
> permit you to investigate both kit and separate purchase options.
>
> regards,
>
> Fred Monson
>
> Frederick C. Monson, PhD
> Center for Advanced Scientific Imaging
> West Chester University
> West Chester, Pennsylvania, USA, 19383
> 610-738-0437
> fmonson@wcupa.edu
>
> ----------
> From: Walzer Susan
> Sent: Wednesday, March 27, 2002 8:14 AM
> To: Histonet (E-mail)
> Subject: Digital Camera
>
> My pathologist is in the market for a new camera, is there a good one that
> can attach to a microscope? (digital) His old one was 35mm. Thanks!
>
>
>
----------------------------------------------------------------------
Date: 27 Mar 2002 09:15:35 -0600
From: Philip Oshel
Subject: Re: Digital Camera
The Nikon Coolpix 950/990/995 series is happily used by many
microscopy types. These cameras have been replaced by the Coolpix
5000.
Adapters are available from most microscopy dealers. One outfit I
like is Optem:
http://www.thales-optem.com/
no financial interest, I just like the products. There are others,
and I don't know all of them, so check around.
Phil
>My pathologist is in the market for a new camera, is there a good one that
>can attach to a microscope? (digital) His old one was 35mm. Thanks!
- --
Philip Oshel
Supervisor, BBPIC microscopy facility
Department of Animal Sciences
University of Wisconsin
1675 Observatory Drive
Madison, WI 53706 - 1284
voice: (608) 263-4162
fax: (608) 262-5157 (dept. fax)
----------------------------------------------------------------------
Date: 27 Mar 2002 09:21:32 -0600
From: "Cazares, Ruth"
Subject: Pneumatic tubing of surgical specimens
Hello again Histonetters,
Is anyone using Pneumatic tube Systems for surgical specimens? Are
specimens in formalin being sent, or just fresh tissue? And does high
velocity affect the tissues in any way? We are trying to find a faster way
of getting our specimens from the O.R. and since there is already an
existing Tube system we are exploring the possiblity of using it for
Histology specimens if possible.
Any and all information, suggestions etc. is welcome and appreciated.
Ruth Cazares
Swedish Covenant Hospital
Chicago, Illinois
----------------------------------------------------------------------
Date: 27 Mar 2002 09:25:25 -0600
From: Walzer Susan
Subject: Camera
Thanks to all for the great info on cameras. I am forwarding them all to my
doc and will let him browse.
----------------------------------------------------------------------
Date: 27 Mar 2002 09:34:05 -0600
From: "George, Cheryl"
Subject: RE: Calretinin
I'm using the patient tissue as a negative control, not applying the primary
antibody. We use the Dako autostainer and have not had problems with any
other antibodies. The first time this happened, the sample was a cell block
of a pleural fluid, the second time a lung biopsy.
> ----------
> From: Carrie Kyle-Byrne[SMTP:ckbyrne@exelixis.com]
> Sent: Tuesday, March 26, 2002 3:34 PM
> To: George, Cheryl
> Cc: 'HistoNet@Pathology.swmed.edu'
> Subject: Re: Calretinin
>
> what are you using for your negative control and how are you doing your
> staining (manual or automated...which machine)? what type of tissue is
> the
> patient specimen?
> carrie
>
> ----- Original Message -----
> From: "George, Cheryl"
> To:
> Sent: Tuesday, March 26, 2002 10:57 AM
> Subject: Calretinin
>
>
> > Hi out there,
> > We do calretinin on human FFPE tissue and over the last couple of
> weeks
> > have had two cases where the patient negative control tissue stains just
> as
> > brightly and specifically as the positive. Other markers on the same
> > patient tissue don't react this way and I was wondering if anyone has an
> > idea about what could cause this.
> >
> > Thanks in advance!!
> >
> > Cheryl
> >
> > Cheryl George, BS, HT (ASCP)
> > Anatomic Pathology Supervisor
> > Elliot Hospital Laboratory
> > One Elliot Way
> > Manchester, NH 03103
> > (603) 663-2686
> >
> >
> >
> >
> >
>
----------------------------------------------------------------------
Date: 27 Mar 2002 09:47:44 -0600
From: "George, Cheryl"
Subject: RE: Calretinin
Pretreatment is the Dako low pH HIER solution for 20 minutes with a 20
minute cool down prior to any staining. Cheryl
> ----------
> From: Bonnie P Whitaker[SMTP:Bonnie.P.Whitaker@uth.tmc.edu]
> Sent: Tuesday, March 26, 2002 4:20 PM
> To: Carrie Kyle-Byrne
> Cc: George Cheryl; 'HistoNet@Pathology.swmed.edu'
> Subject: Re: Calretinin
>
> Also, what pretreatment? Are the other markers pretreated the same way?
> Bonnie Whitaker
> UT Houston
>
> ----- Original Message -----
> From: Carrie Kyle-Byrne
> Date: Tuesday, March 26, 2002 2:34 pm
> Subject: Re: Calretinin
>
> > what are you using for your negative control and how are you doing
> > yourstaining (manual or automated...which machine)? what type of
> > tissue is the
> > patient specimen?
> > carrie
> >
> > ----- Original Message -----
> > From: "George, Cheryl"
> > To:
> > Sent: Tuesday, March 26, 2002 10:57 AM
> > Subject: Calretinin
> >
> >
> > > Hi out there,
> > > We do calretinin on human FFPE tissue and over the last
> > couple of weeks
> > > have had two cases where the patient negative control tissue
> > stains just
> > as
> > > brightly and specifically as the positive. Other markers on the
> > same> patient tissue don't react this way and I was wondering if
> > anyone has an
> > > idea about what could cause this.
> > >
> > > Thanks in advance!!
> > >
> > > Cheryl
> > >
> > > Cheryl George, BS, HT (ASCP)
> > > Anatomic Pathology Supervisor
> > > Elliot Hospital Laboratory
> > > One Elliot Way
> > > Manchester, NH 03103
> > > (603) 663-2686
> > >
> > >
> > >
> > >
> > >
> >
> >
> >
>
----------------------------------------------------------------------
Date: 27 Mar 2002 09:48:06 -0600
From: DGoodwin@CHSNJ.org
Subject: RE: Pneumatic tubing of surgical specimens
Dear Ruth:
We use a pneumatic tube to send surgical specimens that are in formalin. We
have invested in screw-top containers, and the specimens are double -bagged
at the source.
Good luck!
Diana Goodwin, HT
Trenton, NJ
> ----------
> From: Cazares, Ruth[SMTP:RCazares@schosp.org]
> Sent: Wednesday, March 27, 2002 10:10 AM
> To: 'histonet@pathology.swmed.edu'
> Subject: Pneumatic tubing of surgical specimens
>
> Hello again Histonetters,
>
> Is anyone using Pneumatic tube Systems for surgical specimens? Are
> specimens in formalin being sent, or just fresh tissue? And does high
> velocity affect the tissues in any way? We are trying to find a faster
> way
> of getting our specimens from the O.R. and since there is already an
> existing Tube system we are exploring the possiblity of using it for
> Histology specimens if possible.
>
> Any and all information, suggestions etc. is welcome and appreciated.
>
> Ruth Cazares
> Swedish Covenant Hospital
> Chicago, Illinois
>
This electronic transmission and any attachments hereto contains material
that is private and confidential in nature and may be protected by law. It
is for the intended recipient only. Any other disclosures are unintended.
If you have received this transmission, or a copy thereof, in error you are
instructed to immediately and permanently delete this transmission and any
attachments, and to notify the sender of such error by telephone at (609)
394-4000. Thank you for your
cooperation and assistance
----------------------------------------------------------------------
Date: 27 Mar 2002 09:55:42 -0600
From: HornHV@archildrens.org
Subject: RE: Pneumatic tubing of surgical specimens
We do not use our tube specimens for our histology specimens. Formalin is
not allowed in our tube system. We have received the rare fresh specimen
through the tube and it was ok. Our OR has runners that bring the
specimens to us. The clinics deliver their own specimens.
Hazel
> -----Original Message-----
> From: Cazares, Ruth [SMTP:RCazares@schosp.org]
> Sent: Wednesday, March 27, 2002 9:10 AM
> To: 'histonet@pathology.swmed.edu'
> Subject: Pneumatic tubing of surgical specimens
>
> Hello again Histonetters,
>
> Is anyone using Pneumatic tube Systems for surgical specimens? Are
> specimens in formalin being sent, or just fresh tissue? And does high
> velocity affect the tissues in any way? We are trying to find a faster
> way
> of getting our specimens from the O.R. and since there is already an
> existing Tube system we are exploring the possiblity of using it for
> Histology specimens if possible.
>
> Any and all information, suggestions etc. is welcome and appreciated.
>
> Ruth Cazares
> Swedish Covenant Hospital
> Chicago, Illinois
----------------------------------------------------------------------
Date: 27 Mar 2002 10:18:37 -0600
From: "Mottishaw, Brad"
Subject: RE: Pneumatic tubing of surgical specimens
We are utilizing a Pneumatic tube system to transport tissue, and body
fluids to and from the O.R. and the floors here at University of Utah
Medical Center. We performed a validation of the system using tissue to be
dumped, and out dated blood products from multiple sites in the hospital and
from building out of the hospital that use the same tube system (ie..
Huntsman Cancer Institute, Moran Eye Center, and Primary Childrens
Hospital). We used fresh and formalin fixed tissue. We encountered no
problems with the system, just the resistance of some people to try
something new. It is a great time saver especially for frozen sections.
Best of luck,
Brad M. Mottishaw HT(ASCP)
Teaching Coordinator
Anatomical Pathology
A.R.U.P.
University of Utah Medical Center
500 Chipeta way
Salt Lake City, UT 84108
(801)-585-6282
mottisb@aruplab.com
- -----Original Message-----
From: Cazares, Ruth [mailto:RCazares@schosp.org]
Sent: Wednesday, March 27, 2002 8:10 AM
To: 'histonet@pathology.swmed.edu'
Subject: Pneumatic tubing of surgical specimens
Hello again Histonetters,
Is anyone using Pneumatic tube Systems for surgical specimens? Are
specimens in formalin being sent, or just fresh tissue? And does high
velocity affect the tissues in any way? We are trying to find a faster way
of getting our specimens from the O.R. and since there is already an
existing Tube system we are exploring the possiblity of using it for
Histology specimens if possible.
Any and all information, suggestions etc. is welcome and appreciated.
Ruth Cazares
Swedish Covenant Hospital
Chicago, Illinois
----------------------------------------------------------------------
Date: 27 Mar 2002 10:19:07 -0600
From: rueggp
Subject: Re: Calretinin
Cheryl,
I am wondering if you could be getting some antibody carry over from the
DAKO
probe or check the program carefully to see that the right vial for your
neg.
is
being accessed.
Patsy
"George, Cheryl" wrote:
> I'm using the patient tissue as a negative control, not applying the
primary
> antibody. We use the Dako autostainer and have not had problems with any
> other antibodies. The first time this happened, the sample was a cell
block
> of a pleural fluid, the second time a lung biopsy.
>
> > ----------
> > From: Carrie Kyle-Byrne[SMTP:ckbyrne@exelixis.com]
> > Sent: Tuesday, March 26, 2002 3:34 PM
> > To: George, Cheryl
> > Cc: 'HistoNet@Pathology.swmed.edu'
> > Subject: Re: Calretinin
> >
> > what are you using for your negative control and how are you doing your
> > staining (manual or automated...which machine)? what type of tissue is
> > the
> > patient specimen?
> > carrie
> >
> > ----- Original Message -----
> > From: "George, Cheryl"
> > To:
> > Sent: Tuesday, March 26, 2002 10:57 AM
> > Subject: Calretinin
> >
> >
> > > Hi out there,
> > > We do calretinin on human FFPE tissue and over the last couple of
> > weeks
> > > have had two cases where the patient negative control tissue stains
just
> > as
> > > brightly and specifically as the positive. Other markers on the same
> > > patient tissue don't react this way and I was wondering if anyone has
an
> > > idea about what could cause this.
> > >
> > > Thanks in advance!!
> > >
> > > Cheryl
> > >
> > > Cheryl George, BS, HT (ASCP)
> > > Anatomic Pathology Supervisor
> > > Elliot Hospital Laboratory
> > > One Elliot Way
> > > Manchester, NH 03103
> > > (603) 663-2686
> > >
> > >
> > >
> > >
> > >
> >
----------------------------------------------------------------------
Date: 27 Mar 2002 10:26:27 -0600
From: Tim Webster
Subject: BBC Rapid Fix
Hi Everybody,
BBC sells "Rapid Fix". "Rapid fix tm is a fast-acting fixative designed
specifically to be used at the grossing station. It fixes fatty tissues
more rapidly than 10%NBF."
Three questions:
1) Does anybody use it, and what do you think?
2) Suggestions for a study to confirm that no structural or artefactural
changes occur. - Something quick and easy please!
3) Any suggestions other than "Patience is a virtue"!
Background: There are times when we suffer with tissue (human) that need to
be reprocessed due to poor infiltration. I suspect that the problem occurs
when the tissue is inadequately fixed before processing. (It may be the
dehydration, but it occurs mostly on tissue that is either fatty or a little
too fresh, and everythin' else is just peachy! - Hence, I'm looking at the
fixation)
Also, with regard to recent discussions on vendors and reps etc, Adrian from
BBC has been very helpful. We are unlikely to be a huge account, but he has
been friendly and accomadating. However, I would like the opinion of those
in the trenches!
Thanks, and have a good day.
Tim
Tim Webster
Histology Specialist
Northwestern Medical Center
St Albans, VT 05478
(802) 524-1070
twebster@nmcinc.org
----------------------------------------------------------------------
Date: 27 Mar 2002 10:32:21 -0600
From: "Lott, Robert"
Subject: Overstained GMS
Below is a treatment that really works, but it is best to go really slow and
check microscopically over and over until the desired result is obtained.
1. De-coverslip sections and run then back to distilled water.
2. Place section(s) in coplin jar containing 1 part of 1% potassium
"ferri"cyanide to 3 parts distilled water (0.25% potassium
ferricyanide)
3. Monitor continuously with the microscope until excess silver has been
removed.
4. Wash well in distilled water.
5. Re-counterstain as dersired.
Robert L. Lott, HTL(ASCP)
Baptist Health System
800 Montclair Road
Birmingham, AL 35213
205-592-5388
205-592-5646 (fax)
----------------------------------------------------------------------
Date: 27 Mar 2002 11:03:18 -0600
From: Kristen Johnson
Subject: Job Opening - Histology Supervisor
LifeSpan BioSciences Inc., a Seattle-based molecular pathology company
located in downtown Seattle overlooking Elliott Bay is seeking a Histology
Supervisor to supervise and coordinate the workload of the Histology
Technicians, cut paraffin sections conduct H+E staining, run special
techniques to include frozen sectioning and special stains, assist with the
development of writing standard operating procedures, process tissues, embed
and maintain equipment, process and fix tissue dehydrates and gross in
tissue, and act as a liaison with other divisions.
This position requires a minimum of 3-4 years histology experience;, a BS in
a related field, HT/HLT Certification preferred; supervisory experience;
experience with microtome, embedding center, tissue processor, microscope,
crytostat, microtome blades, slide over, auto stainer and drill press;
ability to work well in a team; and strong communication, interpersonal and
problem resolution skills .
We offer a competitive salary, excellent benefits and a dynamic work
environment. For more information please visit our web site at www.lsbio.com
. Qualified candidates are encouraged to send a
cover letter indicating position title and resume to:
LifeSpan BioSciences, Inc.
Human Resources
2401 Fourth Avenue, Suite 900
Seattle, WA 98121
Fax: (206) 464-1723
Email: hr@lsbio.com
LifeSpan BioSciences, Inc. is an Equal Opportunity Employer
----------------------------------------------------------------------
Date: 27 Mar 2002 11:32:12 -0600
From: Garza-Williams.Sara@tchden.org
Subject: RE: Pneumatic tubing of surgical specimens
Ruth,
My institution uses a pneumatic tube system for all specimens (short of
those that won't fit). We send fresh specimens, blood, fluids including
those in formlin through the system. What we do is insure the specimen will
not bounce around in the tube by using foam inserts or towels. So far we
have not had any problems. It has worked great for us because we do all
frozen intra-operative (STAT) consults in the histology lab which is located
one floor up and down a hall from surgery, saves a lot of time!
Hope this inf. helps.
Sara A. Williams
Anatomic Pathology Supervisor
- -----Original Message-----
From: Cazares, Ruth [mailto:RCazares@schosp.org]
Sent: Wednesday, March 27, 2002 8:10 AM
To: 'histonet@pathology.swmed.edu'
Subject: Pneumatic tubing of surgical specimens
Hello again Histonetters,
Is anyone using Pneumatic tube Systems for surgical specimens? Are
specimens in formalin being sent, or just fresh tissue? And does high
velocity affect the tissues in any way? We are trying to find a faster way
of getting our specimens from the O.R. and since there is already an
existing Tube system we are exploring the possiblity of using it for
Histology specimens if possible.
Any and all information, suggestions etc. is welcome and appreciated.
Ruth Cazares
Swedish Covenant Hospital
Chicago, Illinois
CONFIDENTIALITY NOTICE: The information contained in this message is legally
privileged and confidential information intended only for the use of the
individual or entity named above. If the reader of this message is not the
intended recipient, or the employee or agent responsible to deliver it to
the intended recipient, you are hereby notified that any release,
dissemination, distribution, or copying of this communication is strictly
prohibited. If you have received this communication in error, please notify
the author immediately by replying to this message and delete the original
message.
Thank you.
----------------------------------------------------------------------
Date: 27 Mar 2002 12:02:28 -0600
From: "Weems, Joyce"
Subject: RE: Grocott Methenamine Silver
Visit a beauty salon and get some left over perm solution. It will remove
excess silver. Also, a very weak solution of household bleach will work, but
may loosen the tissue more. Then it's the float with the glass rod trick.
Joyce Weems
Pathology Manager
Saint Joseph's Hospital of Atlanta
-----Original Message-----
From: Dudecare@aol.com [SMTP:Dudecare@aol.com]
Sent: Wednesday, March 27, 2002 7:18 AM
To: jqb7@cdc.gov; histonet@pathology.swmed.edu
Subject: Re: Grocott Methenamine Silver
Yes, if the silver is way overdone, and there is no replacement
tissue, what
do you do??
----------------------------------------------------------------------
Date: 27 Mar 2002 13:47:26 -0600
From: Boneslides@aol.com
Subject: Goldner's Trichrome
Many, many moons ago, I used to do a Goldner's trichrome on PMMA sections.
I
have a researcher asking for that stain, and, of course, I can't find a
procedure anywhere. Does anyone have a protocol that they can share? I
would greatly appreciate any help!!
Thanks,
Diane Mahovlic, HT(ASCP)
The Cleveland Clinic Foundation
Cleveland, Ohio
----------------------------------------------------------------------
Date: 27 Mar 2002 15:17:50 -0600
From: "Monson, Frederick C."
Subject: RE: Goldner's Trichrome
OK BonesSlides,
Here's
Goldner's Stain: p337 in Gray (below)
Soln 0: Weigert, 1904: (A)HOH 95 + FeCl3 0.6g; (B) 1% hematoxylin in 95%
EtOH. Make Soln 0 with equal parts (A) and (B)
Soln A: HOH 100 + .07g Ponceau 2R + acid fuchsin 0.03 + HOAc 0.2ml
Soln B: 1% HoAc
Soln C: HOH 100 + phosphomolybdic 4 + Orange G 2
Soln D: HOH 100 + Light Green 0.2g + HOAc 0.2ml
Method:
Soln 0 'till colored then tapwater to blue
Soln A: 5min and into Soln B
Wash
Soln C: 'til collagen colored and into Soln B
Rinse
Soln D: 5min and into Soln B 5min and blot
Absolute EtOH for minimum time
Xylene to Balsam
Result: general cytoplasm red, RBC's orange, collagen green
That's what the book says. You must REALLY want this one.
Regards,
Fred Monson
Frederick C. Monson, PhD
Center for Advanced Scientific Imaging
West Chester University
West Chester, Pennsylvania, USA, 19383
610-738-0437
fmonson@wcupa.edu
P.S. How have you survived without the following book?
BOOK #: 202473 ISBN #: 0-88275-247-2
>
> AUTHOR: GRAY
>
> TITLE: MICROTOMIST'S FORMULARY AND GUIDE
>
> PRICE: 84.50 REFRL/ARRGM TYPE: CLOTH
>
> Shipping $5.00 UPS
>
> AT PRSENT WE ARE UPDATING OUR LISTS AND PORTIONS OF LOOKUP ARE OFFLINE.
>
> THE MICROTOMIST'S FORMULARY AND GUIDE
> Peter Gray,
> 0-88275-247-2
>
> Pages: 808, Binding: Cloth,
>
> Description:
> This is a known and recognized source reference work. The book includes a
> treatise on the art of making microscopic slides from biological
> specimens,
> as well as a classified list of the formulas and techniques used in this
> art.
>
>
URL: http://www.krieger-publishing.com/
Krieger Publishing P.O. Box 9542
Melbourne, Florida U.S.A. 32902-9542
Phone (321) 724-9542 #225# Fax (321) 951-3671
Suffering from old-age and hockey fever at the same time is HELL!!! Go
North America!!!!
> ----------
> From: Boneslides@aol.com
> Sent: Wednesday, March 27, 2002 2:30 PM
> To: histonet@pathology.swmed.edu
> Subject: Goldner's Trichrome
>
> Many, many moons ago, I used to do a Goldner's trichrome on PMMA sections.
> I
> have a researcher asking for that stain, and, of course, I can't find a
> procedure anywhere. Does anyone have a protocol that they can share? I
> would greatly appreciate any help!!
>
> Thanks,
>
> Diane Mahovlic, HT(ASCP)
> The Cleveland Clinic Foundation
> Cleveland, Ohio
>
>
----------------------------------------------------------------------
Date: 27 Mar 2002 18:33:11 -0600
From: Melissa Jensen
Subject: Paraffin
We have used our microwave to melt paraffin from tissue slides..to speed up
the turn around time for recuts.3-4 mins with a 700 watt...Our old microwave
bit the dust. We have a new one 1000 watt..I have set the power to 70% and
used the 3-4 min thing...No luck..Tried it at 3-4-5- mins..also at full
power..I cant find a consistent setting...any advise! Its either fried or
paraffin gloss.
Thanks
******************* NOTE *******************
There may be important message content
contained in the following MIME Information.
********************************************
- ------------------ MIME Information follows ------------------
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- --Boundary_(ID_RTX2E6omGFnzBdgZ0xhoaw)
Content-type: text/plain; charset=iso-8859-1
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Content-type: text/html; charset=iso-8859-1
Content-transfer-encoding: 7BIT
We have used our microwave to melt paraffin
from
tissue slides..to speed up the turn around time for recuts.3-4 mins with a
700
watt...Our old microwave bit the dust. We have a new one 1000 watt..I have
set
the power to 70% and used the 3-4 min thing...No luck..Tried it at 3-4-5-
mins..also at full power..I cant find a consistent setting...any advise! Its
either fried or paraffin gloss.
Thanks
- --Boundary_(ID_RTX2E6omGFnzBdgZ0xhoaw)--
----------------------------------------------------------------------
Date: 27 Mar 2002 19:03:06 -0600
From: Shona Penhale
Subject: TH and paraffin
I am hoping there is someone out there who has used
Tyrosine Hydroxylase with paraffin embedded peripheral
nervous tissue. I am trying to test erectile tissue
in the female for confirmation of nerve type. My
tissue is from cadavers, they sit a few days prior to
embalming and then after 2 months in the cooler I can
harvest tissue. I am hoping to find a simple,
non-flourescent) protocol to test known nerve tissue.
Thanks for any help!
Shona
__________________________________________________
Do You Yahoo!?
Yahoo! Movies - coverage of the 74th Academy AwardsAE
http://movies.yahoo.com/
----------------------------------------------------------------------
Date: 27 Mar 2002 19:49:13 -0600
From: Phyllis Davie
Subject: FW: Paraffin
- ------ Forwarded Message
From: Phyllis Davie
Date: Wed, 27 Mar 2002 17:32:04 -0800
To: Melissa Jensen
Subject: Re: Paraffin
I1m not sure this helps, but it may be due to this:
A 700-watt microwave operates at 700 watts for the whole time you set it.
A 1000-watt microwave, set at 70%, operates at 1000 watts for 70% of the
time.
Microwave magnetrons pretty much have 2 settings, ON and OFF. So while
adjusting the % power may adjust the overall energy 3dose2 you give to your
sections, it will be coming in boluses of 1000 watts or nothing. Therefore,
it may not be possible to entirely reproduce your earlier performance.
Sorry this isn1t really an answer. Maybe other more savvy people can help
you out.
Best of luck,
Phyllis Davie
PhenoPath Laboratories--Seattle, WA
pdavie@phenopath.com
on 3/27/02 4:18 PM, Melissa Jensen at melzy@indytel.com wrote:
> We have used our microwave to melt paraffin from tissue slides..to speed
up
> the turn around time for recuts.3-4 mins with a 700 watt...Our old
microwave
> bit the dust. We have a new one 1000 watt..I have set the power to 70% and
> used the 3-4 min thing...No luck..Tried it at 3-4-5- mins..also at full
> power..I cant find a consistent setting...any advise! Its either fried or
> paraffin gloss.
> Thanks
>
>
>
> ------ End of Forwarded Message
******************* NOTE *******************
There may be important message content
contained in the following MIME Information.
********************************************
- ------------------ MIME Information follows ------------------
> This message is in MIME format. Since your mail reader does not understand
this format, some or all of this message may not be legible.
- --Boundary_(ID_Z1Yvb+rXYTGfO8hSLdhr+g)
Content-type: text/plain; charset=ISO-8859-1
Content-transfer-encoding: QUOTED-PRINTABLE
<<<<<< See above "Message Body" >>>>>>
- --Boundary_(ID_Z1Yvb+rXYTGfO8hSLdhr+g)
Content-type: text/html; charset=ISO-8859-1
Content-transfer-encoding: 7BIT
FW: Paraffin
- ------ Forwarded Message
From: Phyllis Davie <pdavie@phenopath.com>
Date: Wed, 27 Mar 2002 17:32:04 -0800
To: Melissa Jensen <melzy@indytel.com>
Subject: Re: Paraffin
I’m not sure this helps, but it may be due to this:
A 700-watt microwave operates at 700 watts for the whole time you set
it.
A 1000-watt microwave, set at 70%, operates at 1000 watts for 70% of the
time.
Microwave magnetrons pretty much have 2 settings, ON and OFF. So while
adjusting the % power may adjust the overall energy “dose” you
give to your sections, it will be coming in boluses of 1000 watts or
nothing.
Therefore, it may not be possible to entirely reproduce your earlier
performance.
Sorry this isn’t really an answer. Maybe other more savvy people
can help you out.
Best of luck,
Phyllis Davie
PhenoPath Laboratories--Seattle, WA
pdavie@phenopath.com
on 3/27/02 4:18 PM, Melissa Jensen at melzy@indytel.com wrote:
We have used our
microwave to melt paraffin from tissue slides..to speed up the turn around
time for recuts.3-4 mins with a 700 watt...Our old microwave bit the dust.
We
have a new one 1000 watt..I have set the power to 70% and used the 3-4 min
thing...No luck..Tried it at 3-4-5- mins..also at full power..I cant find a
consistent setting...any advise! Its either fried or paraffin gloss.
Thanks
- ------ End of Forwarded Message
- --Boundary_(ID_Z1Yvb+rXYTGfO8hSLdhr+g)--
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