Pretreatment is the Dako low pH HIER solution for 20 minutes with a 20
minute cool down prior to any staining.  Cheryl

> ----------
> From: 	Bonnie P Whitaker[SMTP:Bonnie.P.Whitaker@uth.tmc.edu]
> Sent: 	Tuesday, March 26, 2002 4:20 PM
> To: 	Carrie Kyle-Byrne
> Cc: 	George Cheryl; 'HistoNet@Pathology.swmed.edu'
> Subject: 	Re: Calretinin
> 
> Also, what pretreatment?  Are the other markers pretreated the same way?
> Bonnie Whitaker
> UT Houston
> 
> ----- Original Message -----
> From: Carrie Kyle-Byrne 
> Date: Tuesday, March 26, 2002 2:34 pm
> Subject: Re: Calretinin
> 
> > what are you using for your negative control and how are you doing 
> > yourstaining (manual or automated...which machine)?  what type of 
> > tissue is the
> > patient specimen?
> > carrie
> > 
> > ----- Original Message -----
> > From: "George, Cheryl" 
> > To: 
> > Sent: Tuesday, March 26, 2002 10:57 AM
> > Subject: Calretinin
> > 
> > 
> > > Hi out there,
> > >    We do calretinin on human FFPE tissue and over the last 
> > couple of weeks
> > > have had two cases where the patient negative control tissue 
> > stains just
> > as
> > > brightly and specifically as the positive.  Other markers on the 
> > same> patient tissue don't react this way and I was wondering if 
> > anyone has an
> > > idea about what could cause this.
> > >
> > > Thanks in advance!!
> > >
> > > Cheryl
> > >
> > > Cheryl George, BS, HT (ASCP)
> > > Anatomic Pathology Supervisor
> > > Elliot Hospital Laboratory
> > > One Elliot Way
> > > Manchester, NH 03103
> > > (603) 663-2686
> > >
> > >
> > >
> > >
> > >
> > 
> > 
> > 
>