RE: Burned tissue update question

From:Ralph Marcucio


Any chance of getting a picture of this for us?  I am doing in situs
(radioactive) and use an emulsion to detect the signal.  In anumber of cases
the epithelium (any epithelium) harborts what looks like air bubbles (or
dirt) after coverslipping----as if they have been overdried and unwettable.


Ralph Marcucio, PhD
Department of Orthopaedic Surgery
University of California at San Francisco
San Francisco, Ca 94143

Phone: 415-502-4945

-----Original Message-----
Sent: Wednesday, March 20, 2002 4:00 PM
Subject: Re: Burned tissue update question


Your problem is not reagent grade alcohol.I have used reagent grade alcohol
in many of the hospitals that I have worked and it worked fine. It maybe in
the three 100% alcohols in your program. This many alcohols will take the
bond water out off the tissue causing problems, such as,  dry, burnt
looking tissue. Try cutting down to two 100% alcohols and adding a 95%
alcohol in its place.

Hope this helps.

Mari Ann Mailhiot BA HT.
Applications Specialist
Technical Assistance Center
Leica Microsystems

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