Steve-

Any chance of getting a picture of this for us?  I am doing in situs
(radioactive) and use an emulsion to detect the signal.  In anumber of cases
the epithelium (any epithelium) harborts what looks like air bubbles (or
dirt) after coverslipping----as if they have been overdried and unwettable.

Ralph

Ralph Marcucio, PhD
Department of Orthopaedic Surgery
University of California at San Francisco
San Francisco, Ca 94143

Phone: 415-502-4945
Fax:415-476-1128


-----Original Message-----
From: mari.ann.mailhiot@leica-microsystems.com
[mailto:mari.ann.mailhiot@leica-microsystems.com]
Sent: Wednesday, March 20, 2002 4:00 PM
To: CrochiereSteve@aol.com
Cc: histonet@pathology.swmed.edu
Subject: Re: Burned tissue update question



Steve,

Your problem is not reagent grade alcohol.I have used reagent grade alcohol
in many of the hospitals that I have worked and it worked fine. It maybe in
the three 100% alcohols in your program. This many alcohols will take the
bond water out off the tissue causing problems, such as,  dry, burnt
looking tissue. Try cutting down to two 100% alcohols and adding a 95%
alcohol in its place.

Hope this helps.

Mari Ann Mailhiot BA HT.
Applications Specialist
Technical Assistance Center
Leica Microsystems