RE: Burned tissue update question

From:

Cynthia Favara

My inclination would be to shorten up on the time in the 100% alcohol.

Good luck

Cynthia Favara

-----Original Message-----
From: CrochiereSteve@aol.com [mailto:CrochiereSteve@aol.com]
Sent: Tuesday, March 19, 2002 4:49 PM
To: histonet@pathology.swmed.edu
Subject: Burned tissue update question

I think I may have a problem with the reagent alcohol. This is my new processing schedule. Any suggestions would be welcome:
NBF 2 hrs
70% 15 min
80% 30 min
95% 30 min
100% 45 min X3
Histosolve for 40 min X3
Paraffin for 30, 40, 40, then sit until embedded in the 4th change

I use reagent alcohol rather than Ethanol because of the taxes. Even though I work in a hospital, the Path dept. is a private entity, so cannot quickly get a tax free aproval.

s

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