Nile Blue Sulfate vital staining

From:Ralph Marcucio


I have been trying to detect cell death in whole embryos using Nile Blue
sulfate, and to date, I have had no luck, even in my very positive controls.
Does anyone have a protocol for using Nile Blue Sulfate to for vital

Thanks in advance,

Ralph Marcucio, PhD
Department of Orthopaedic Surgery
University of California at San Francisco
San Francisco, Ca 94143

Phone: 415-502-4945
Fax: 415-476-1128

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