Hi-

I have been trying to detect cell death in whole embryos using Nile Blue
sulfate, and to date, I have had no luck, even in my very positive controls.
Does anyone have a protocol for using Nile Blue Sulfate to for vital
staining??

Thanks in advance,
Ralph

Ralph Marcucio, PhD
Department of Orthopaedic Surgery
University of California at San Francisco
San Francisco, Ca 94143

Phone: 415-502-4945
Fax: 415-476-1128