Mouse Brains

From:Lee & Peggy Wenk

If you have experience fixing/processing mouse brains, and would be willing
to talk with a researcher at my hospital, please email me at my WORK EMAIL

Let me know your name, phone number and email address.

I received a telephone call from a doctor doing research on mouse brains at
our research building. They have been perfusing the mouse, but now want to
remove the brain and then fix. (Don't know why the change in protocol. I
got the message late last night.) He needs advise from me.

- what % formalin
- buffered or unbuffered formalin
- how long - minimum, maximum
- best processing schedule - times, %, etc.

I could make a semi-"intelligent" guess, extrapolating from what we do with
whole human brains, but . . . mice are not human, and we all know that
handling animal tissue IS different than handling human tissue.

So again, if you are willing to be a mouse brain resource for me, please
email me at work today, so I can give him your name, phone number and email

Thank you in advance.

Peggy A. Wenk, HTL(ASCP)SLS
William Beaumont Hospital
Royal Oak, MI 48073

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