We are having a problem with prostate needle biopsies for high molecular 
weight cytokeratin.  Most are fixed in Bouin's, some in formalin, and 
paraffin embedded.  We have tried using only DI water in the waterbath (with 
no additives), using silanized slides, and draining the slides well before 
drying them in a 60 degree oven for 1-2 hours.  They often are washing during 
deparaffinizing, some during steamer pretreatment with HipH target retrieval. 
 Is anyone else having this problem?  What have you found that works for you?

Ellen Yee
Sacramento