immuno on mouse tissue

From:Cathy Gorrie

For all the immunohistologists out there.

This is a pretty general question about immunohistology on mouse 
tissue. When using monoclonal antibodies, what special measures need 
to be taken, or what secondaries need to be used, in order to get 
specific staining. I can imagine if you use an anti-mouse secondary 
everything in the tissue will be targeted, or at least a lot of 
background from serum proteins, immuno cells etc.


Can you not use monoclonals on mouse tissue? I have always used other 
species of animal, or human and this has never been a problem before.


Is this not as big a problem as I imagine it could be?

Thanks for your help,

Cathy Gorrie
Scientific Officer
Neural Injury Research Unit,
School of Medical Sciences,
University of New South Wales
Sydney, N.S.W. 2052

Phone: 61-2-9385 2462
Fax   : 61-2-9313 6252

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