fixation of smears

From:Melissa Jans

I decided to repost this question in light of all the
histonet problems we have been having.
What is the best method for fixing cytological smears
that will be stained for Amyloid, GMS, or AFB?  Should
they be air dried first and then fixed?  Should they
be fixed in ethanol, methanol or formalin?  Thanks for
your help!
Melissa Jans
Lead Scientist, Histology lab
University of Iowa Healthcare

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